愈痫灵颗粒对戊四氮致痫大鼠海马神经生长因子与认知功能的影响

目的 :探讨愈痫灵颗粒对痫性大鼠海马区神经生长因子(nerve growth factor, NGF)及认知功能的影响。 方法 :选取150只清洁级SD大鼠,随机选取10只作为正常组;其余动物用戊四氮(PTZ)造模。将符合模型标准的大鼠随机分为4组,ig给药,即愈痫灵颗粒组、丙戊酸钠组、拉莫三嗪组给药剂量分别为810 mg ·kg^-1,122 mg ·kg^-1,10.1 mg ·kg^-1,模型组、正常组每天给予同等体积蒸馏水。每天1次,连续给药28 d后,用开场实验(open field test)测定大鼠认知功能变化,采用免疫组织化学方法观察NGF的表达。 结果 :与模型组相比,愈痫灵颗粒能显著提高戊四氮致痫大鼠的开场实验成绩,差异有显著统计学意义( P <0.01);愈痫灵颗粒能提高NGF阳性细胞平均灰度值和降低NGF阳性细胞平均吸光度,差异有显著统计学意义( P <0.01)。 结论 :降低致痫大鼠海马区NGF的表达是愈痫灵颗粒改善戊四氮致痫大鼠认知功能的可能机制之一。

Effect of Yuxianling Granules on the NGF of Hippocampus and Cognitive Function in Rats with Epilepsy Induced by Pentylenetetrazol

Objective: To study the effect of Yuxianling granule (YXLG) on the hippocampal nerve growth factor (NGF) and cognitive function in epileptic rats. Method: 150 SD rats were used for the experiment, and 10 of them were randomly selected as the blank control group. The rest animals were treated with pentylenetetrazol (PTZ) to establish the model. The rats consisting with the model standard were divided randomly into 4 groups, including model group, YXLG group, sodium valproate group and lamotrigine group. . Using gavage administration, the drug doses were 810 mg ·kg^-1,122 mg ·kg^-1,10.1 mg ·kg^-1 accordingly. The model group and control group were given the same volume of distilled water daily. All the treatment was 1 times daily, lasted for 28 d. The animals were tested by open field test for measuring changes in cognitive function. Immunohistochemical method was employed to observe the expression of NGF. Result: Compared with the model group, YXLG could increase the opening test results in rats with pentylenetetrazol-induced epilepsy, the difference was statistically significant ( P <0.01). YXLG could enhance the average gray value of NGF-positive cells and reduce the average optical density of NGF-positive cells, the difference was statistically significant ( P <0.01). Conclusion: The reason that YXLG improves the cognitive function of the epileptic rats may be related with the reduced expression of NGF in epileptic rats.