葡萄糖及胰岛素对牛血管内皮细胞凋亡的影响

目的通过对牛血管内皮细胞（ＥＣ）的体外培养研究糖尿病（ＤＭ）状况下葡萄糖（Ｇｌｕ）、胰岛素（Ｉｎｓ）对ＥＣ凋亡的影响。方法吖啶橙／溴乙锭（ＡＯ／ＥＢ）荧光染色及ＤＮＡ片段的琼脂糖凝胶电泳对ＥＣ凋亡定性，ＥＬＩＳＡ方法测定ＤＮＡ片段定量ＥＣ凋亡。结果高浓度Ｇｌｕ（２０ｍｍｏｌ／Ｌ、４０ｍｍｏｌ／Ｌ）和Ｉｎｓ（３００ｍＵ／Ｌ、３０００ｍＵ／Ｌ）能够诱发ＥＣ发生凋亡。ＥＣ凋亡与Ｇｌｕ和Ｉｎｓ浓度成正比，并且为时间依赖性。低浓度Ｉｎｓ（３０ｍＵ／Ｌ）明显改善Ｇｌｕ４０ｍｍｏｌ／Ｌ下所致ＥＣ的凋亡，但高浓度Ｉｎｓ与Ｇｌｕ有正性协同作用，增加ＥＣ凋亡。高浓度甘露醇（Ｍａｎ２０ｍｍｏｌ／Ｌ、４０ｍｍｏｌ／Ｌ）不引起ＥＣ明显凋亡。结论ＤＭ环境下高血糖、高Ｉｎｓ血症能诱发ＥＣ凋亡，促进ＤＭ血管并发症的发生、发展

Effect of different concentrations of glucose and insulin on apoptosis of bovine vascular endothelial cells

Objective To study the effects of different concentrations of glucose and insulin on apoptosis of cultured endothelial cells (ECs). Methods Apoptasis of ECs was studied qualitatively with acridine orange(AO)/ethidium bromide(EB) fluorescence staining and DNA agarose gel electrophoresis and quantitatively with ELISA method. Results High concentrations of glucose and insulin induced apoptosis. Apoptosis of ECs was positively correlated with concentrations of glucose and insulin and was time dependent. Low concentration of insulin decreased apoptosis of ECs induced by glucose but high concentrations of insulin had synergistic action with glucose and increased apoptosis of ECs. High concentrations of mannitol did not induce obvious apoptosis of ECs. Conclusion High plasma glucose and insulin levels in diabetes mellitus may induce opoptosis of vascular endothelium and accelerate the development of vascular complications.