A simple and improved method for diagnosis of tuberculosis using hypertonic saline and sodium hydroxide to concentrate and decontaminate sputum |
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| Authors: | Ricaldi Jessica N Guerra Humberto |
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| Institution: | Instituto de Medicina Tropical Alexander von Humboldt, Av. Honorio Delgado 430, Lima 31, Republic of Peru. jricaldi@ucsd.edu |
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| Abstract: | We report the use of hypertonic saline and sodium hydroxide (HS-SH) for concentration-decontamination of sputum samples for the diagnosis of tuberculosis. This method increases detection of mycobacteria in smears compared to examination of direct smears, is comparable and reduces contamination in cultures compared to NALC-NaOH. |
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