| 拉曼被孢霉γ-亚麻酸高产突变株的选育 |
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| 引用本文： | 李植峰,张玲,赖炳森,沈晓京,谭亚芳,孙树秦.拉曼被孢霉γ-亚麻酸高产突变株的选育[J].中国生化药物杂志,2001,22(3):129-131. |
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| 作者姓名： | 李植峰 张玲 赖炳森 沈晓京 谭亚芳 孙树秦 |
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| 作者单位： | 北京军医学院生化教研室, |
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| 基金项目： | 军队九五医药卫生科研基金课题(96D069) |
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| 摘 要： | 目的诱变、筛选γ 亚麻酸 (GLA)高产菌株。方法采用 5 氟尿嘧啶、紫外线、氯化锂复合诱变及自然分离的方法 ,对拉曼被孢霉AS 3.34 13进行诱变筛选。结果获得一株GLA高产突变株F5。该菌株GLA产量较原始菌株提高了 30 0 %。传代实验证明该菌株遗传性质稳定。经对其发酵培养基及发酵条件的优化 ,该菌株GLA生产能力可达 115 3.6 3mg/L ,较原始菌株提高 335 .87%。结论拉曼被孢霉GLA高产突变株F5已达工业生产菌株要求。
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| 关 键 词： | 拉曼被孢霉 γ-亚麻酸 诱变 筛选 |
| 文章编号： | 1005-1678(2001)03-0129-03 |
| 修稿时间： | 2000年9月25日 |
Breeding of highly
productive |
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| LI Zhi-feng,ZHANG Ling,LAI Bing-sen,SHEN Xiao-Jing,TAN Ya-fang,SUN Shu-qin.Breeding of highly
productive[J].Chinese Journal of Biochemical Pharmaceutics,2001,22(3):129-131. |
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| Authors: | LI Zhi-feng ZHANG Ling LAI Bing-sen SHEN Xiao-Jing TAN Ya-fang SUN Shu-qin |
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| Abstract: | Purpose The aim is to breed a highly productive γ-linolenic acid mutant strain.Methods The original strain Mortierella ramanniance AS 3.3413 was treated by 5-FU,UV,LiCl as mutanting agent.Results A highly productive γ-linolenic acid mutant strain F5 was successfully obtained.The γ-linolenic acid yield of mutant strain F5 was increased by 300% as compared with that of the original strain.Pass-generation test showed that the heredity character of mutant strain F5 was stable.After the optimal medium and culture condition was established, the yield of γ-linolenic acid was up to 1 153.63 mg/L,and was increased by 335.87% as compared with that of the original strain.Conclusion The mutant strain can be used for the industrial production of GLA. |
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| Keywords: | Mortierella ramanniance γ-linolenic acid Mutant Breeding |
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