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| 柴胡对人乳头瘤病毒杀灭作用的实验研究 | |
| 引用本文： | 李劲,罗奎章,林奕,陈闻纳,赖穗萍. 柴胡对人乳头瘤病毒杀灭作用的实验研究[J]. 中国中西医结合皮肤性病学杂志, 2005, 4(3): 171-173 |
| 作者姓名： | 李劲 罗奎章 林奕 陈闻纳 赖穗萍 |
| 作者单位： | 1. 广东省深圳市东湖医院皮肤科,深圳,518020 2. 广州中山大学附属第一医院皮肤科,广州,510080 3. 广州市东山区人民医院,广州,510080 |
| 摘 要： | 目的筛选柴胡抗人乳头瘤病毒(HPV)的有效成分.方法用溶媒(石油醚、乙醚、乙酸乙酯、正丁醇、乙醇、蒸馏水)对柴胡分别进行提取,应用荧光定量聚合酶链反应技术(FQ-PCR)对各提取物进行体外抗病毒药效学实验,检测离体尖锐湿疣皮损中HPV-DNA的扩增情况.结果HPV-DNA经柴胡水提物作用后,其PCR扩增检测为阴性,最低有效浓度为0.2g/mL,而用其他有机溶剂提取部分PCR扩增检测为阳性.结论柴胡的水提成分有抗人乳头瘤病毒作用. |
| 关 键 词： | 柴胡 尖锐湿疣 人乳头瘤病毒 聚合酶链反应技术 |
| 文章编号： | 1672-0709（2005）03-0171-03 |
| 收稿时间： | 2005-01-27 |
| 修稿时间： | 2005-03-23 |
Laboratory Study on Anti-human Papillomavirus Activity of Bupleurum Chinese |
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| LI Jin,LUO Kui-zhang,LIN Yi,CHEN Wen-na,LAI Sui-ping. Laboratory Study on Anti-human Papillomavirus Activity of Bupleurum Chinese[J]. Chinese J of Dermatovenerology Integr Tradit and West Med, 2005, 4(3): 171-173 | |
| Authors: | LI Jin LUO Kui-zhang LIN Yi CHEN Wen-na LAI Sui-ping |
| Abstract: | Objective To screen anti-human papillomavirus(HPV) effective fraction from Bupleurum Chinese. Methods Bupleurum Chinese was extracted with systematic solvents (petroleum benzin, ether, ethyl acetate, n-butanol, ethyl alcohol and distilled water) respectively. The fluoresence quantitative polymerase chain reaction(FQ-PCR) was applied in vitro pharmacodynamics for the extracts from Bupleurum Chinese. Than we examined the amplification of HPV-DNA in the lesions of the isolated condyloma acuminatum. Results After the water extracts of Bupleurum Chinese reacted with HPV-DNA, the PCR amplification showed negative with the minimum effective concentration 0.2 g/mL. But the other organic solvents extracts from Bupleurum Chinese were positive. Conclusion The water extracts from Bupleurum Chinese can take effect on anti-human papillomavirus. |
| Keywords: | Bupleurum Chinese condyloma acuminatum human papillomavirus polymerase chain reaction |
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