Survivin在非小细胞肺癌中的表达及其与bcl-2、p53表达的关系

目的　探讨Survivin在非小细胞肺癌组织 (non smallcelllungcancer ,NSCLC)中的表达 ,及其与bcl 2、p5 3蛋白表达的相关性。方法　应用免疫组织化学SP法 ,检测Survivin、bcl 2、p5 3蛋白在 5 7例NSCLC组织和 10例正常肺组织中的表达。结果　Survivin蛋白在正常肺组织中不表达 ,5 7例NSCLC组织中 ,3 4例表达阳性 ,占 5 9.6%。Survivin蛋白表达与分化程度、淋巴结转移密切相关 (P<0 .0 5 )。NSCLC组织bcl 2蛋白表达阳性、阴性组中 ,Survivin蛋白阳性表达率分别为 78.9%( 15 / 19)和 5 0 %( 19/ 3 8) ,两者比较 ,差异有显著性 (P <0 .0 5 ) ;p5 3蛋白表达阳性、阴性组中 ,Survivin蛋白阳性表达率分别为 72 .2 %( 2 6/ 3 6)和 3 8.1%( 8/ 2 1) ,两者比较 ,差异有显著性 (P <0 .0 5 ) ,Survivin蛋白表达与bcl 2、p5 3蛋白表达密切相关。结论　Survivin在NSCLC组织中表达上调 ,通过抑制细胞凋亡 ,在NSCLC的发生和发展中起到重要作用 ;凋亡相关基因bcl 2的上调和抑癌基因p5 3的失活与Survivin的表达可能在NSCLC癌变中起协同作用

Expression of Survivin and Its Relationship with Expression of bcl-2,p53 in Non-small-cell Lung Cancer

Objective To study the expression of Survivin in non-small-cell lung cancer and its relationship with the expression of bcl-2, p53 proteins. Methods Expression of the Survivin,bcl-2,p53 proteins was evaluated by immunohistochemical streptavidin-biotin peroxidase (SP) method in 57 non-small-cell lung cancer tissues and 10 normal lung tissues. Results Survivin was expressed in 34 of 57(59.6%) cases of non-small-cell lung cancer. In contrast, normal lung tissue did not express Survivin. Overexpression of Survivin was related to the differentiation and lymph node metastasis. Survivin positive cases were strongly associated with bcl-2 expression (78.9% versus 50%, P <0.05) and p53 expression (72.2% versus 38.1%, P <0.05).Conclusions Up-regulation of Survivin expression in non-small-cell lung cancer suggests that Survivin may play an important role in the oncogenesis and progression of non-small-cell lung cancer by the inhibition of apoptosis. Survivin,up-regulation of apoptosis related gene bcl-2,de-activation of antioncogene p53 may play synergetic roles in process of carcinogenesis of non-small-cell lung cancer.