Dicer is required for chromosome segregation and gene silencing in fission yeast cells |
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Authors: | Provost Patrick Silverstein Rebecca A Dishart David Walfridsson Julian Djupedal Ingela Kniola Barbara Wright Anthony Samuelsson Bengt Radmark Olof Ekwall Karl |
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Affiliation: | Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, S-171 77 Stockholm, Sweden Europe. |
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Abstract: | RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (dcr1+) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into approximately 23 nucleotide fragments in vitro, and dcr1Delta cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif. |
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