| 雄性小鼠肾虚对其雄性仔鼠睾丸组织氧化应激水平的影响 |
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| 引用本文： | 孙洁,周安方,周艳艳,方婷.雄性小鼠肾虚对其雄性仔鼠睾丸组织氧化应激水平的影响[J].中华中医药杂志,2009(2). |
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| 作者姓名： | 孙洁 周安方 周艳艳 方婷 |
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| 作者单位： | 浙江省中医院;湖北中医学院; |
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| 基金项目： | 湖北省自然科学基金资助课题(No.2004ABA184) |
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| 摘 要： | 目的:观察雄性小鼠肾虚对其雄性仔鼠睾丸氧化应激水平的影响。方法:将30只雄性昆明小鼠用计算机取随机数法随机分为正常组、模型组和补肾组3组。其中模型组和补肾组采用房劳加惊恐的复合伤肾法制造肾精亏虚模型。补肾组每天给予0.16ml/10g体质量的补肾填精方浓缩液灌胃。正常组及模型组予等量生理盐水灌胃,造模并给药21d。从造模结束次日起,所有小鼠与正常发情雌鼠配对饲养5d后,取出雌鼠待其分娩。仔鼠饲养至6周龄时每组随机取出10只雄性仔鼠,取出左侧睾丸,检测其组织匀浆上清中丙二醛(malondialdehyde,MDA)、过氧化物歧化酶(superoxide dismutase,SOD)活性、总抗氧化能力(total antioxygen capacity),T-AOC。结果:模型组仔鼠睾丸组织MDA水平(24.26±6.05)μmol/gprot明显高于正常组(16.54±1.47)μmol/gprot及补肾组(15.04±1.50)μmol/gprot,(P<0.01)。模型组仔鼠睾丸组织T-AOC水平(2.71±0.55)U/mgprot与正常组仔鼠(4.33±1.54)U/mgprot比较明显降低(P<0.0...
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| 关 键 词： | 肾精亏虚 动物模型 子代 补肾填精方 氧化应激 |
Effect of Kidney-jing Deficiency on oxidative stress of male offspring |
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| SUN Jie,ZHOU An-fang,ZHOU Yan-yan,FANG Ting.Effect of Kidney-jing Deficiency on oxidative stress of male offspring[J].China Journal of Traditional Chinese Medicine and Pharmacy,2009(2). |
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| Authors: | SUN Jie ZHOU An-fang ZHOU Yan-yan FANG Ting |
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| Institution: | SUN Jie1,ZHOU An-fang2,ZHOU Yan-yan2,FANG Ting2(1Zhejiang Hospital of TCM,Hangzhou 310006,China,2Hubei College of TCM,Tanhualin,Wuhan 430061,China) |
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| Abstract: | Objective:To investigate the effects of kidney-jing deficiency of male mice on the oxidative stress of their male offspring. Methods:Thirty 6-weeks-old Kunming male mice were divided into three groups randomly:the blank control group,the model group and the kidney-tonifying group. The model and the kidney-tonifying groups were stressesd by fear plus excessive sex to establish a kidney-jing deficiency model,and meanwhile the latter were given concentrated solution of Kidney-tonifying recipe intragastrically ... |
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| Keywords: | Kidney-jing deficiency Animal model Offspring Oxidative stress Kidney-tonifying Recipe |
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