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Ion-pair liquid chromatographic assay of angiotensin-converting enzyme activity |
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| Authors: | Maurich V Pitotti A Vio L Mamolo M G |
| Affiliation: | Istituto di Tecnica Farmaceutica, Università di Trieste, 34100 Trieste, Italy. |
| Abstract: | A rapid and specific high-performance liquid chromatographic assay for the quantitative determination of angiotensin-converting enzyme activity is described. Hippuryl-L-histidyl-L-leucine (Hip-His-Leu) is used as substrate and the released hippuric acid is measured. The procedure is accurate and precise and no extraction is required. |
| Keywords: | Angiotensin-converting enzyme high-pressure liquid chromatographic enzyme assay hippuric acid. |
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