妊娠肝内胆汁淤积症患者胎盘细胞凋亡及调控基因的研究

目的　通过观察妊娠肝内胆汁淤积症 (ICP)患者胎盘细胞凋亡调控基因p5 3、bax和bcl 2在胎盘中的表达 ,探讨细胞凋亡基因在胎盘细胞凋亡中的作用。方法　采用原位末端标记法(TUNEL)和免疫组织化学方法对 3 1例ICP患者 (ICP组 )的胎盘组织中p5 3、bax和bcl 2基因表达及调亡指数进行检测 ,并以 3 1例正常妊娠妇女的胎盘组织作对照 (对照组 )。结果　 (1)ICP组胎盘组织中细胞滋养细胞、合体滋养细胞、蜕膜细胞及间质细胞中细胞凋亡指数分别为 (49 1± 9 1) %、(46 6±9 8) %、(3 5 1± 9 5 ) %、(3 8 7± 9 7) % ,明显高于对照组的 (2 2 5± 6 2 ) %、(2 1 6± 5 2 ) %、(17 9±6 2 ) %、(17 0± 4 7) %。两组比较 ,差异有极显著性 (P <0 0 1)。 (2 )而调控基因p5 3的表达 ,ICP组明显高于对照组 ,两组比较 ,差异有极显著性 (P <0 0 1) ;bax的表达在两组滋养细胞中比较 ,差异无显著性 (P >0 0 5 )。但在合体滋养细胞、蜕膜细胞及间质细胞中 ,ICP组明显高于对照组 ,两组比较 ,差异有极显著性 (P <0 0 1) ;bcl 2的表达在ICP组胎盘组织细胞滋养细胞、合体滋养细胞、蜕膜细胞及间质细胞中 ,均明显低于对照组 (P <0 0 1)。 (3 )p5 3、bax和bcl 2在ICP组合体滋养细胞中的表达分别是 (75 9± 8 2 ) %、(65

Relationship between p53, bax and bcl-2 expression and cell apoptosis in intrahepatic cholestasis of pregnancy

OBJECTIVE: To investigate the changes of gene p53, bax and bcl-2 expression and to study the role on cell apoptosis in placenta through apoptosis in placenta with intrahepatic cholestasis of pregnancy (ICP). METHODS: TdT-mediated dUTP nick end labeling (TUNEL) and immuno-histochemistry methods were used for 31 placental samples with ICP and 31 normal placental samples to detect apoptosis index and the expression of p53, bax and bcl-2 in placenta tissues. RESULTS: The apoptosis index in placental cytotrophoblast, syncytiotrophoblast, decidual cells and mediate cell on ICP group was significantly higher than that in control group (P < 0.01). The expression of p53 was higher than those of control. Bax expression in syncytoitrophoblast, decidual cell and mediate cell in ICP group was higher (P < 0.01), but the expression of bax in placental cytotrophoblast cell was not significantly higher (P > 0.05). The expression of bcl-2 of these positions in ICP group was lower than that in control group (P < 0.01) p53, bax and bcl-2 expressions in (chi +/- s)% in the syncytoitrophoblast cells of placenta with ICP is 75.9 +/- 8.2, 65.8 +/- 13.8, 45.3 +/- 11.3. CONCLUSION: The placental dysfunction may closely relate to the apoptosis. Abnormal overexpression of p53, bax and the low expression of bcl-2 on placental tissue of ICP was the main reason of placental apoptosis.