NR1和GluR2在成年大鼠海马结构的共表达

目的探讨NMDA受体NR1亚单位(NMDA/NR1)和AMPA受体GluR2亚单位(AMPA/GluR2)在正常成年大鼠海马结构的分布特征。方法在多聚甲醛固定的海马切片上,采用免疫荧光组织化学方法染色,通过激光扫描共聚焦显微镜观察NR1和GluR2在正常成年大鼠海马结构中的表达与分布。结果NR1和GluR2在正常成年大鼠海马结构广泛表达,并且两者免疫荧光染色阳性表达的分布基本一致,其中在CA1和CA3区的锥体细胞层以及齿状回门区的中间神经元表达最强;在CA1和CA3区的分子层以及齿状回的颗粒细胞层中也有明显的NR1和GluR2免疫荧光染色颗粒的分布;但在CA3区的分子层和齿状回的多形层NR1的表达阳性强于GluR2。阳性反应主要位于神经细胞的胞膜和突起。结论NMDA/NR1和AMPA/GluR2在海马结构中的分布特征基本一致,提示它们可能共同参与完成海马的某些重要功能。

The Expressions and Distribution Characters of Glutamate Receptor Subunits NR1 and GluR2 in the Hippocampal Formation of Adult Rats

Objective To investigate the expressions and distributions of N-methyl-D-aspartic acid NMDA/NR1 and alpha-amino-3-hydroxy-5-methyl-4 isoxazolepropionic acid AMPA/GluR2 subunits in the hippocampal formation.Methods The immunofluorescence histochemical staining was made on paraformaldehyde fixed hippocampal tissue sections for then observed by confocal laser scanning microscopy.Results There were widely positive expressions and basically same distributions of NR1 and GluR2 in the hippocampal formation of adult rats,especially in pyramidal layer of CA1 and CA3 regions and in the interneurons of hilus fascia dentata.Positive expressions,which mainly existed in the cytomembrane and processes of the neurons,also distributed in molecular layer of CA1 and CA3 regions as well as in granule layer of dentate gyrus.However,the immunoreactive intensity of NMDA/NR1 was stronger than that of AMPA/GluR2 in molecular layer of CA3 area and in polymorphic layer of dentate gyrus.Conclusion The distributions of NMDA/NR1 and AMPA/GluR2 in the hippocampal formation are basically same,this suggests that they may execute cooperatively some important tasks.