| 反相HPLC法测定全血中环孢素A的血药浓度 |
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| 引用本文： | 黄健,戴志凌,张明雄.反相HPLC法测定全血中环孢素A的血药浓度[J].中国药房,2000,11(5):220-221. |
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| 作者姓名： | 黄健 戴志凌 张明雄 |
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| 作者单位： | 昆明医学院第二附属医院药剂科,昆明市 |
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| 摘 要： | 目的 :建立一种用反相HPLC法测定全血中环孢素A的血药浓度的分析方法。方法 :反相HPLC ,YWG -C18 柱 ,流动相为乙腈 -甲醇 -异丙醇 -水 (57∶24∶1∶18) ,流速 :1 0ml/min ,柱温 :71℃ ,检测波长212nm。结果 :环孢素A浓度在40 2μg/L～1286 4μg/L范围内有良好的线性关系 ,相关系数r=0 997。平均回收率为98 04 % ,RSD为0 6 % ,n=9。日内差RSD≤5 4 %(n=5) ,日间差RSD≤6 2 % (n=5)。结论 :此法简便、稳定 ,可应用于移植病人的CsA血药浓度监测。
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| 关 键 词： | 反相HPLC法 环孢素A 血药浓度监测 |
| 文章编号： | 1001-0408(2000)05-0220-02 |
| 修稿时间： | 2000年8月20日 |
Determination of the Contents of Cyclosporine A in Human Whole Blood by RP-HPLC |
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| HUANG Jian,DAI Zhiling,ZHANG Mingxiong,QIU Lizhen,CUI Lan.Determination of the Contents of Cyclosporine A in Human Whole Blood by RP-HPLC[J].China Pharmacy,2000,11(5):220-221. |
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| Authors: | HUANG Jian DAI Zhiling ZHANG Mingxiong QIU Lizhen CUI Lan |
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| Abstract: | OBJECTIVE:To develop a method to determine the contents of cyclosporine A in human whole blood METHO_DS:Detection was performed on YWG-C18 column with acetonitrile-methanol-isopropand-water(57∶24∶1∶18),as mobile phase Detecting wavelength was 212nm; The flow rate was 1 01ml/min and column temperature was 71℃ RESULTS:The calibration curve was linear in the concentrations ranged from 40 2μg/L to 1 286 4μg/L(r=0 997) The average recovery was 98 04% with RSD of 0 6%(n=9) The RSD within day was less than 5 4%(n=5) and the RSD between days was less than 6 2%(n=5) CONCLUSION:This method is simple and reliable and can be applied to the monitoring of blood drug concentration in patients receiving transplantation |
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| Keywords: | RP-HPLC cyclosporine A monitoring of drug concentration in blood |
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