Overnight ovarian tissue transportation for centralized cryobanking: a feasible option |
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Authors: | Jana Liebenthron Markus Montag Jochen Reinsberg Maria Köster Vladimir Isachenko Katrin van der Ven Hans van der Ven Jan-Steffen Krüssel Michael von Wolff |
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Institution: | 1. UniCareD, University Cryobank for Assisted Reproductive Medicine and Fertility Protection at UniKiD, University Women’s Hospital Düsseldorf, Moorenstr. 5, Düsseldorf 40225, Germany;2. Ilabcomm GmbH, Eisenachstr. 34, Augustin 53757 St., Germany;3. Department of Gynecological Endocrinology and Reproductive Medicine, University Clinic Bonn, Sigmund-Freud-Str. 25, Bonn 53125, Germany;4. KWZ Germany GmbH, Joseph-Schumpeter-Allee 1, Bonn 53227, Germany;5. Department of Gynecological Endocrinology and Reproductive Medicine, University Clinic Cologne, Kerpener Straße 34, Cologne 50931, Germany;6. MVZ für Frauenheilkunde und IvF-Medizin Bonn GbR, Godesberger Allee 64, Bonn 53175, Germany;7. University Women’s Hospital Bern, Division of Gynecological Endocrinology and Reproductive Medicine, Effingerstrasse 102, Bern 3010, Switzerland |
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Abstract: | Research questionIs overnight transportation of ovarian tissue before cryopreservation in a centralized cryobank from the FertiPROTEKT network feasible?DesignData from 1810 women with cryopreserved ovarian tissue after overnight transportation from December 2000 to December 2017 were analysed with a focus on transportation, tissue activity parameters and pregnancy, and delivery rates after transplantation.ResultsA total of 92.4% of tissue samples arrived at ideal temperatures of 2–8°C, 0.4% were transported at temperatures lower than ideal and 6.4% were transported at temperatures that were too high, generally due to mishandling of the inlayed cool packs of the transportation boxes. In 62 women, 78 tissue transplantations were carried out. A subgroup of 30 women who underwent a single orthotopic transplantation with fulfilled criteria of a complete follow-up after transplantation until the end of study, a premature ovarian insufficiency after gonadotoxic therapy as well as the absence of pelvic radiation, was further analysed. In this group, transplantations into a peritoneal pocket accounted for 90%. Transplants were still active at 1 year and above after transplantation in 93.3%. Pregnancy and delivery rates were 46.7% and 43.3%, respectively, with one ongoing pregnancy at the end of the study.ConclusionsOvernight transportation for central cryobanking is a feasible concept that results in high reproducible success rates through standardized professional tissue freezing and storage. |
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Keywords: | Centralized cryobanking Fertility preservation Gonadotoxic therapy Ovarian tissue cryopreservation Ovarian tissue transplantation Overnight transportation |
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