豚鼠胆囊SP及CGRP免疫反应神经纤维的光镜和电镜观察

采用ABC免疫组织化学和电镜观察方法 ,对豚鼠胆囊P物质 (SP)和降钙素基因相关肽 (CGRP)免疫组化反应阳性神经纤维及突触小泡的超微结构特点进行了研究。结果显示 ,豚鼠胆囊含有SP和CGRP免疫反应阳性的神经纤维和肥大细胞 ,以及SP免疫反应阳性神经元。这些神经纤维束是被神经膜细胞完全或不完全包裹的无髓神经纤维 ,其神经纤维内含有的突触小泡形态和大小不一 ,电镜下将其分为 3种类型 :①以小型无芯小泡为主 ,并有少量大型有芯小泡。②以小型有芯小泡为主 ,并有少量无芯小泡和大型有芯小泡。③以大型有芯小泡为主 ,并有少量小型无芯小泡。豚鼠胆囊的神经纤维内 ,散在分布的突触小泡多为第 3种。但在血管、淋巴管周围的SP和CGRP免疫反应阳性神经纤维内的突触小泡多为小型有芯小泡。提示豚鼠胆囊可接受SP和CGRP等肽能神经的支配。

Observation of SP and CGRP Immunoreactive Characteristics in Gallbladder of Guinea Pig with Electron Microscope

To investigate the biological behavior of the gallbladder, observed the histological pattern of nerve fiber, neuron and mast cell by using the ABC immunohistochemical method. Light electron microscope was used in order to observe synaptic vesicle in the nerve fiber of guinea pig gallbladder, a number of large dense cored vesicles and a few small undense cored vesicles were seen in the nerve fiber containing substance P and calcitonin gene-related peptide (CGRP) positive immunoreactivity. The experiment also showed that around the blood vessel and lymph vessel the synaptic vesicles of positive immunoreactivity nerve fiber were small dense cored vesicles.