Selection of medium for serum-free primary culture of adult rat hepatocytes |
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Authors: | M Miyazaki L Y Bai J Sato |
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Affiliation: | Division of Pathology, Okayama University Medical School, Japan. |
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Abstract: | To select a suitable medium for serum-free primary culture of adult rat hepatocytes, ten commercially-available synthetic media were compared for their ability to maintain the cells under serum-free and serum-supplemented conditions with special reference to attachment, survival and albumin secretion. It was found that Williams' medium E and DM-160 medium were the best among the ten media for maintaining hepatocytes under serum-free conditions in primary culture. |
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