大鼠急性坏死性胰腺炎时P物质在肠壁上的表达及其与肠黏膜通透性的关系

目的:了解急性坏死性胰腺炎(ANP)时大鼠回肠组织中P物质的表达及其与肠黏膜通透性的关系。方法:健康成年SD大鼠随机分为对照组40只和ANP组40只,对照组开腹后只翻动胰腺,ANP组大鼠胰胆管恒速逆行注射5%牛黄胆酸钠制成ANP大鼠模型,分别于制模成功后8、12、16和20 h处死大鼠采取标本。用同位素法测定肠黏膜通透性,免疫组织化学方法检测P物质在回肠组织中的表达水平和组织学定位。结果:ANP组大鼠回肠组织中P物质的表达以及肠黏膜通透性(99mTc-DT-PA排泄率)较对照组明显增加(P<0.05)。P物质在回肠组织中的表达与肠黏膜通透性呈明显正相关(r=0.66,P<0.01)。结论:ANP时大鼠回肠组织中P物质表达升高,且随时间延长而增加,其表达与大鼠肠黏膜通透性呈正相关。

Expression of substance P in intestinal tissue of rats and the relation between substance P and mucosa permeability in acute necrotizing pancreatitis

Objective To investigate the expression of substance P in rats' intestinal tissue and study the relation between substance P and mucosa permeability in acute necrotizing pancreatitis(ANP).Methods 80 adult Sprague-Dawley rats were randomly divided into control group((n=)40) and ANP group((n=)40).Sacrifices were made in 8,12,16 and 20?h later in every group after ANP model was established by injecting 5% sodium taurocholate into pancreatic duct respectively.The intestinal mucosal permeability and the expression of substance P were studied.Results In ANP groups,intestinal mucosal permeability and the expression of substance P were significantly higher than those in the control group((P<)0.05).The overexpression of substance P was correlated with the intestinal mucosal permeability in ANP(r=0.66,P<(0.01)).Conclusion The expression of substance P and the permeability in intestine of rats are increased significantly in ANP.There exists a possitive correlation between substance P and the intestinal permeability.