黄芩素对缝隙连接蛋白Cx32组成的GJIC功能的影响

目的:在转染并稳定表达Cx32的HeLa细胞上,观察黄芩素对Cx32组成的GJIC及Cx32蛋白表达水平的影响。方法:细胞接种荧光示踪法观察不同浓度黄芩素对GJIC的影响;细胞免疫荧光法观察黄芩素在影响GJIC功能浓度范围内对Cx32蛋白表达的影响。结果:黄芩素(2.5μmol/L,5μmol/L,10μmol/L)增强Cx32组成的GJIC功能的荧光渗透率为10.04%,22.5%和35.33%;黄芩素(2.5μmol/L,5μmol/L,10μmol/L)增强组成GJIC的Cx32蛋白表达的荧光强度为(27.12±0.30)%,(56.31±1.02)%和(83.47±1.85)%。结论:黄芩素可增强缝隙连接蛋白Cx32组成的GJIC功能,其机制可能与增加Cx32蛋白表达水平有关。

The effect of baicalein on the function of gap junction intercellular communication composed by Cx32

Objective：To investigat the effect of baicalien on gap junction intercellular communication and the expression of Cx32 protein in Cx32-transfected HeLa cells. Methods：The function of GJIC was determined by＂parachute＂dye-coupling assay. Immunofluorescence assay was used to detect the expression of Cx32 protein located in the membrane of HeLa cells. Results：＂Parachute＂ assay results showed that the intercellular dye coupling through gap junction composed of Cx32 increased by 10.04%,22.5%and 35.33% when the cells exposed to ellagic acid of 2.5 μmol/L,5 μmol/L and 10 μmol/L. Immunofluorescence assay results showed that fluorescence intensity for the expression of Cx32 protein located in membrane could be enhanced by （27.12±0.30）%,（56.31±1.02）%and （83.47±1.85）%when cells were exposed to baicalein of 2.5μmol/L,5 μmol/L and 10 μmol/L. Conclusion：Baicalein can enhance the function of gap junction in Cx32-transfected HeLa cells,probably through increasing the Cx32 expression.