Studies of Monoclonal Antibodies Specific for Major Histocompatibility Complex Products of the Rat |
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| Authors: | S. HEMMI M. FENNER H. BINZ K. WINTERHALTER H. WIGZELL |
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| Affiliation: | Institute for Immunology and Virology, University of Zürich, Zürich, Switzerland and Laboratory for Biochemistry, Swiss Federal Institute for Technology, Zürich, Switzerland, and Department of Immunology, Karolinska Institute, Stockholm, Sweden |
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| Abstract: | Sixteen monoclonal anti-idiotypic antibodies were prepared against RT1 antigen-specific monoclonal antibodies by means of an inhibition assay. Three (110-3, 112-89, and 133-4) were produced against antibody 3-8C2 (19), three (178-60, 181-14, and 184-38) against antibody 11/23/5, and three (144-3, 144-33, and 146-31) against antibody 1-5A4. One monoclonal anti-idiotypic antibody (21-39) was directed against antibody 1-8D2 and six (CE1-3, CE2-1, CE7-2, CE8-46, CE9-35, and CE10-2) against antibody 3-12A1. The anti-idiotypic antibodies were all highly specific for the respective monoclonal antibody used for the induction of the anti-idiotype and did not cross-react with other monoclonal antibodies. |
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