| 气-质联用结合衍生化法分析血浆中甲基苯丙胺和氯胺酮 |
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| 引用本文： | 王皓玉,王玉瑾,贾娟,曹洁,尉志文,贠克明. 气-质联用结合衍生化法分析血浆中甲基苯丙胺和氯胺酮[J]. 中国医院药学杂志, 2011, 0(4) |
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| 作者姓名： | 王皓玉 王玉瑾 贾娟 曹洁 尉志文 贠克明 |
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| 作者单位： | 山西医科大学法医学院; |
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| 基金项目： | 山西省自然科学基金资助项目(编号:20011066,2007011105); “十一五”国家科技支撑计划项目(编号:2007BAK26B05); 山西医科大学博士启动基金资助项目(编号:03200814) |
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| 摘 要： | 目的:考察家兔血浆中甲基苯丙胺和氯胺酮同时衍生化后GC/MS检测分析结果。方法:血浆样品中加入内标物丙基解痉素(SKF525A)后碱化,乙醚萃取,三氟醋酸酐(TFA)衍生化,GC/MS全扫描定性、内标法和工作曲线法定量分析。结果:甲基苯丙胺和氯胺酮在血浆中的线性检测范围分别为0.010 0~10.0μg.mL·1和0.010 0~20.0μg.mL·1;方法回收率为95.26%~101.10%;日内及日间相对标准差均小于15%。结论:建立了衍生化后同时测定血浆中甲基苯丙胺及氯胺酮的GC/MS定性定量分析方法,该法简便、灵敏、重复性好,适用于甲基苯丙胺和氯胺酮混合滥用中毒案例的快速鉴定。
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| 关 键 词： | 甲基苯丙胺 氯胺酮 衍生 气-质联用法 |
Simultaneous determination of methamphetamine and ketamine in plasma by GC/MS with derivation |
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| WANG Hao-yu,WANG Yu-jin,JIA Juan,CAO Jie,WEI Zhi-wen,YUN Ke-ming. Simultaneous determination of methamphetamine and ketamine in plasma by GC/MS with derivation[J]. Chinese Journal of Hospital Pharmacy, 2011, 0(4) |
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| Authors: | WANG Hao-yu WANG Yu-jin JIA Juan CAO Jie WEI Zhi-wen YUN Ke-ming |
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| Affiliation: | WANG Hao-yu,WANG Yu-jin,JIA Juan,CAO Jie,WEI Zhi-wen,YUN Ke-ming (School of Forensic Medicine,Shanxi Medical University,Shanxi Taiyuan 030001,China) |
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| Abstract: | OBJECTIVE To establish a method for simultaneous determination of methamphetamine and ketamine in plasma derivatized with TFA by GC/MS.METHODS After adding internal standard(IS) SKF525A,plasma samples were alkalized,extracted with ether and derivatized with trifluoroacetic anhydride(TFA).Optimized qualitative analysis employed full-scan mode and quantitative analysis by internal standard method and calibration curves by GC/MS.RESULTS The linear range for methamphetamine and ketamine in plasma were 0.010 0-1... |
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| Keywords: | methamphetamine ketamine derivation GC/MS |
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