难治性癫痫患者脑组织胶质原纤维酸性蛋白和多药耐药基因蛋白的免疫电镜观察

目的在电镜水平上观察多药耐药基因蛋白(MDR1蛋白)和胶质原纤维酸性蛋白(GFAP)在难治性癫痫患者脑组织的表达，确定其在细胞超微结构中的定位。方法临床难治性癫痫患者手术切除的病理组织标本，固定、冷冻超薄切片，以免疫胶体金(PAG)标记技术标记检测MDR-1和GFAP蛋白，在电镜下观察其表达。结果MDR-1蛋白与GFAP均在胶质细胞内有阳性表达，见于胶质细胞膜及其胞浆的广泛区域。在神经元和毛细血管内皮细胞中未见有GFAP和MDR-1蛋白的阳性表达。结论在难治性癫痫患者脑组织中，MDR1蛋白与GFAP的高表达现象只存在于胶质细胞胞膜和胞浆内。

IMMUNOGOLD LOCALIZATION OF GFAP AND MDR1 IN SURGICALLY RESECTED BRAIN TISSUES FROM CLINICAL INTRACTABLE EPILEPSY PATIENTS BY TRANSMISSION ELECTRON MICROSCOPY

Objective To determine the ultrastructural localization of MDR1 and GFAP in the surgically resected brain tissues from intractable epilepsy patients. Methods Expression of MDR1 and GFAP in brain tissues was examined by using PAG immunolabeling technique for electron microscopy. Results The MDR1 and GFAP labeled by gold particles were only detected at some reactive astrocytes. The positive gold particles were mainly located in the astrocytic cytoplasm and their membrane, but not in the nucleus.Conclusion The expression of MDR1 and GFAP in the brain of patients with clinically intractable epilepsy were mainly located at the cytoplasm and membrane of certain reactive astrocytics.;