注射用丹红对大鼠血液流变学及血栓、血小板功能的影响

目的观察注射用丹红对血液流变学及血栓、血小板功能的影响。方法 SD大鼠随机分为6组:对照组、血瘀模型组、注射用丹红(2.5、5.0、10.0 g/kg)组及丹参注射液组(5.0 g/kg)。制备大鼠急性血瘀模型,观察注射用丹红对大鼠血液流变学和血小板黏附和聚集的影响;采用体外血栓形成试验观察注射用丹红抗血栓形成作用。结果注射用丹红5.0 g/kg组可明显降低血瘀模型大鼠血沉、红细胞聚集指数、血沉方程K值;10.0 g/kg组可明显降低血瘀模型大鼠全血黏度(切变率5、1/s),血沉、全血低切还原黏度、红细胞聚集指数、血沉方程K值、全血低切相对黏度。注射用丹红5.0、10.0 g/kg组可抑制血瘀大鼠血小板聚集和黏附,并可使体外血栓长度缩短、干湿重降低。结论注射用丹红的抗缺血作用可能与改善血液流变学、抑制血栓形成、改善血小板黏附和聚集有关。

Influence of Danhong for injection on hemorrheology, thrombus and platelet function in rats

Objective To investigate the influence of Danhong for injection on hemorrheology,thrombus and platelet function,and to explore the evidence for the clinical research.Methods The rats were divided into six groups:control group,model group,Danhong for injection(2.5,5.0,10.0 g/kg)groups,Danshen injection(5.0 g/kg)group.The changes of hemorrheology,rates of platelet aggregation and adhesiveness,antithrombosis in vitro were observed by the acute blood stasis rats model.Results Danhong for injection(5.0 g/kg) could reduce ESR,erythrocyte aggregation index,equation K value of ESR,and Danhong for injection(10.0 g/kg) could reduce viscosity of whole blood(shear rate 5,1/s),ESR,whole blood low shear reductive viscosity,erythrocyte aggregation index,equation K value of ESR,whole blood low shear relativity viscosity.Danhong for injection(5.0,10.0 g/kg)could inhibit the rates of platelet aggregation and adhesiveness,as well as the thrombosis length,dry weight and wet weight in vitro on model rats.Conclusion The results suggest that Danhong injection anti-ischemic effects may be concerned with improving hemorrheology,inhibiting thrombus,rates of platelet aggregation and adhesiveness.