硝基苯和苯胺对大鼠原代肝细胞氧化损伤的作用

目的:从氧化损伤途径研究硝基苯和苯胺对Wistar大鼠原代肝细胞的联合毒作用,为预防环境化合物对人体健康损害提供理论依据。方法:分别用不同浓度(1.25、2.5、5.0、10.0和20～mmol/L),的硝基苯、苯胺及其混合物处理大鼠原代肝细胞24 h后,采用MTT法测定各组细胞的存活率,并进一步测定各组细胞或上清液中丙二醛(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-px)、丙氨酸氨基转移酶(ALT)和超氧化物歧化酶(SOD)的变化。结果:与阴性对照组相比,大鼠原代肝细胞经硝基苯、苯胺及其混合物处理24 h后,其细胞存活率均随所给化合物浓度的增加而降低(P<0.05),且硝基苯-苯胺联合染毒组的细胞存活率明显低于单一化合物的细胞存活率(P<0.05)。随着硝基苯、苯胺和两者混合物浓度的增高,MDA、ALT含量呈现上升趋势(P<0.05);且硝基苯-苯胺联合染毒组细胞MDA、ALT含量均显著高于硝基苯和苯胺单独存在时(P<0.05)。与阴性对照组比较,硝基苯和苯胺及其混合物均可显著降低细胞SOD、GSH和GSH-px含量(P<0.05),并呈现剂量反应关系(P<0.05)。与硝基苯和苯胺单独作用组相比,硝基苯-苯胺联合染毒组细胞SOD、GSH和GSH-px活力均显著降低(P<0.05)。结论:硝基苯和苯胺及其混合物可造成大鼠原代肝细胞氧化损伤,硝基苯与苯胺对肝细胞毒性作用可能存在着协同作用,其作用机制有待进一步研究。

Combined toxicity of nitrobenzene and aniline in primary culture of rat hepatocytes

OBJECTIVE:To study the combined toxicity of nitrobenzene and aniline on primary culture hepatocyte of Wistar rats.METHODS:Nitrobenzene and aniline alone or combined were used to treat the cells for 24 h,and then MTT method was used to measure the survival rate of the cells.Kits were used to measure the MDA,ALT,SOD,GSH and GSH-px levels in the cells or supernatant.RESULTS:After treating with different concentrations of nitrobenzene and aniline alone or combined for 24 h,the survival rate of the cells was significantly lower than that of the cells in control group,with a dose-response relationship.After treating with 10 and 20 mmol/L nitrobenzene and aniline alone or combined for 24 h,the cell MDA and ALT levels of all groups were significantly higher than that of control group(P<0.05).The cell SOD,GSH and GSH-px levels in groups were significantly lower than that of control group(P<0.05).There were significant differences of MDA,ALT,SOD,GSH and GSH-px levels between the combined treatment group and those treated with nitrobenzene or aniline(P<0.05).CONCLUSION:Nitrobenzene and aniline alone or combined may lead to synergistic toxicity effect on hepatocytes.Further research is needed for study.