维吾尔族妇女宫颈癌病理进程与Toll样受体9蛋白表达的关系

目的:Toll样受体9(Toll-like receptor-9,TLR9)是天然免疫受体之一,近期研究表明TLR9与多种上皮性肿瘤发生的早期行为密切相关。本研究拟从蛋白质水平观察宫颈上皮组织中TLR9表达变化与维吾尔族妇女宫颈癌病理进程的关系。方法:收集维吾尔族妇女宫颈炎、宫颈上皮内瘤变cervical intraepithelial neoplasia,CIN)和宫颈鳞癌(cervical squamous cell carcinoma,CSCC)患者的宫颈上皮石蜡包埋组织标本共97例,采用免疫组织化学方法检测各组织中TLR9蛋白表达水平。结果:TLR9蛋白在绝大部分宫颈炎患者的宫颈上皮细胞中不表达(88.2%)或弱表达(11.8%),但是随着宫颈上皮内瘤变和宫颈癌的发生,表达水平逐渐上升,其阳性率依次为慢性宫颈炎(2/17,11.8%),CINⅠ(4/19,28.6%),CINⅡ(3/10,30.0%),CINⅢ(12/24,50.0%),CSCC(17/32,53.1%),5组之间比较差异有统计学意义(P0.05),CINⅢ及CSCC与宫颈炎间的阳性率差异显著(P0.05),但是CINⅠ和CINⅡ与宫颈炎间的差异无统计学意义(P0.05),并且在CSCC中,不同肿瘤分化程度之间其TLR9蛋白阳性率的差异无统计学意义(P0.05)。结论:TLR9蛋白可能参与了宫颈癌的发生和发展的进程。

The association between cervical cancer pathogenesis in Uighur women and Toll-like receptor 9 expression

OBJECTIVE：The aim of this study was to investigate the association of Toll-like receptor 9 protein expression with cervical cancer pathogenesis.METHODS：Levels of TLR9 protein in chronic cervicitis,cervical intraepithelial neoplasia（CIN） and cervico-squamous cell carcinoma（CSCC） tissues from 97 Uighur patients were examined by immunohistochemistry.RESULTS：Immunohistochemical staining showed that TLR9 expression was mainly observed as cytoplasmic staining and was undetectable（88.2%） or weak（11.8%） in chronic cervicitis tissues,gradually increased in accordance with the histopathological grade in the following order：chronic cervicitis（2/17,11.8%）,CINⅠ（4/19,28.6%）,CINⅡ（3/10,30.0%）,CINⅢ（12/24,50.0%）,CSCC（17/32,53.1%）.There were statistical differences among five groups（P 0.05）.The difference was statistically significant for CINⅢand CSCC compared with cervicitis （P 0.05）,but not for CINⅠand CINⅡ（P 0.05）.CONCLUSION：This study showed that the TLR9 protein expression was,to some extent,associated with the development of cervical precancerous lesion and cancer.