环孢素A对兔肺缺血/再灌注损伤的保护作用

目的探讨线粒体通透性转变孔(mPTP)抑制剂环孢素A(CsA)在肺缺血/再灌注损伤(I/R)中的作用以及mPTP作为靶目标肺保护策略的可行性。方法健康家兔40只,随机均分为4组:假手术组、CsA组、低分子右旋糖酐(LMD)组和I/R组。采用原位缺口末端标记(TUNEL)法检测肺组织细胞凋亡情况,免疫组化法检测肺组织细胞色素C(CytC)的含量,电镜下观察肺组织线粒体的破坏情况。结果 CsA组肺组织CytC含量和细胞凋亡指数均高于假手术组(P<0.01),但明显低于LMD组和I/R组,且LMD组的细胞凋亡指数和CytC含量均低于I/R组。结论 CsA能够在再灌注早期抑制mPTP的开放,减少I/R损伤肺组织的细胞凋亡,起到肺保护作用。

Effects of cyclosporine A on protecting lung with ischemia/reperfusion in rabbits

Objective To investigate the effects of cyclosporine A(CsA),a inhibitor of mitochondrial permeability transition pore(mPTP),on lung ischemia/reperfusion(I/R),and explore the viability of lung protection strategy by inhibiting the mPTP.Methods Single lung in vivo I/R animal model was established.40 rabbits were randomly averagely divided into four groups:sham(S) group,cyclosporine A(CsA) group,low molecular dextran(LMD) and I/R group.Apoptosis of lung tissue pneumocyte was assessed by TUNEL method,Cytochrome C(CytC) in lung tissue was detected by immunohistochemcal techniques,the damage of mitochondrial was observed by electron microscope.Results The content of CytC in cytoplasm and the value of apoptosis index(AI) in CsA group were evidently higher than that of S group,but it’s evidently lower than that of LMD group and I/R group,and the value of AI and the content of CytC in LMD group were lower than those of I/R group.Conclusion CsA can inhibit the opening of mPTP in early reperfusion,reduce the apoptosis of lung tissue pneumocyte,and protect the lung against I/R.