叶酸对乙醇诱发的人成淋巴细胞遗传损伤的影响

目的：探讨叶酸（folic acid,FA）对乙醇（ethanol,EtOH）诱发的人成淋巴细胞遗传损伤的影响。方法：人成淋巴细胞株GM12593培养在含有不同FA浓度（20、200、2000 nmol/L）的RPMI-1640培养基中,并同时在每种FA浓度的培养体系中分别加入0.09%、0.36%和1.34%（V/V）的EtOH,8 d后用细胞松弛素B（cytochalasin B,CB）处理28 h,胞质阻断微核细胞组分析（cytokinesisblockmicronucleus cytome assay,CBMN Cyt）比较不同培养条件下的细胞遗传损伤,包括微核化双核细胞（micronucleated binucleatedcell,MNed BNC）、核芽（nuclear bud,BUD）、核质桥（nucleoplasmic bridges,NPB）的频率差异。结果：乙醇在受试细胞中诱发MNedBNC、BUD和NBP频率显著升高（P〈0.01）;2 000 nmol/L的叶酸能显著降低由乙醇诱发的MNed BNC、BUD和NBP频率（P〈0.01）。结论：乙醇对细胞遗传物质具有损伤效应,叶酸对乙醇的遗传毒性具有防护效应。

The effect of folic acid on genetic damage induced by ethanol in human lymphoblast cell line

OBJECTIVE:The study focused on the effect of folic acid(FA) on genetic damage induced by ethanol(EtOH) in human lymphoblast cell line.METHODS:The human lymphoblast cell line GM12593 were cultured in RPMI-1640 medium modified with different concentrations of FA(20,200,2 000 nmol/L).Ethanol was added into the medium at the concentrations of 0.09%,0.36%and 1.34%(V/V) through the culture period.After 8 days culture,the cytokinesis-block micronucleus assay(CBMN) was used to evaluate frequencies of micronucleated binucleated cell(MNed BNC),nuclear buds(BUD) and nucleoplasmic bridges(NPB) in binucleated cells.RESULTS:Ethanol significantly increased the frequencies of MNed BNC,BUD and NPB(P<0.01).A concentration of 2 000 nmol/L of FA reduced the genotoxic effects from ethanol significantly(P <0.01).CONCLUSION:Ethanol could induce DNA damage,folic acid was able to protect human from the genotoxicity induced by enthnol in vitro.