三氯乙烯药疹样皮炎病人外周血c-fos、c-myc、K-ras和p53 mRNA表达水平的研究

目的:探讨三氯乙烯(trichloroethylene,TCE)药疹样皮炎病人外周血癌基因c-fos、c-myc、K-ras、p53 mRNA表达水平的变化情况。方法:分别抽取4例健康人(对照组)及4例三氯乙烯致变态反应病人(病例组)抗凝外周全血,采用实时荧光定量PCR(real-time quantitative PCR)技术检测外周血中c-fos、c-myc、K-ras和p53 mRNA的表达水平。结果:与健康对照者比较,三氯乙烯药疹样皮炎病人外周血c-fos mRNA表达水平升高352%,c-myc mRNA升高41%,K-ras mRNA升高136%,p53 mRNA升高64%,两组间上述基因mRNA表达水平的差异均有统计学意义(P0.01或P0.05)。结论:三氯乙烯药疹样皮炎病人外周血癌基因表达水平增加,提示三氯乙烯可能有一定的致癌风险。

mRNA expression of oncogenes in patients with allergic dermatitis induced by trichloroethylene

OBJECTIVE:To study mRNA expression of oncogenes(c-fos,c-myc,K-ras,p53) in peripheral blood of patients allergic to trichloroethylene(TCE).METHODS:Peripheral blood samples were collected from healthy workers(control group) and allergic patients(case group).Real-time quantitative PCR was applied to detect mRNA expression of oncogenes c-fos,c-myc,K-ras,p53.RESULTS:The level of c-fos mRNA expression increased by 352%in TCE patients when compared with control(P<0.01),c-myc increased by 41%,K-ras by 136%and p53 increased by 64%.mRNA expression levels of these oncogenes showed significant differences between case and control groups(P<0.01 or P<0.05).CONCLUSION:Trichlorcethylene could induce oncogene expression in patients with allergic dermatitis,indicating that TCE might be potentially carcinogenic.