广东省65株耶氏菌的系统鉴定与鼠疫疫源地性质的探讨

目的鉴定65株耶氏菌,了解菌株的生物学特性,抗原抗体与鼠疫菌的相关性,探讨疫源地的现状和性质。方法65株菌用3种致病性耶氏菌诊断血清,分别做玻片、试管凝集试验、生化试验、生长特性,挑选14株菌做毒力因子、毒力基因、致病性、交叉免疫原性、动物试验和血清学、药敏试验。结果 63株小肠结肠炎耶氏菌血清型42株O∶3、21株O∶9,中间型1株,假结核血清II型1株;VW+2株,VW±10株,VW-2株;14株菌F1Ag、F1Ab全阴;小肠结肠炎5株菌均有2个毒力基因(ail、ystA)阳性,VW+和VW±对小白鼠、家兔有致病性,动物感染一种耶氏菌后有交叉免疫力,抗原、活菌交叉吸收鼠疫抗血清后血清学IHA、RIHA没有交叉性,常用抗菌素敏感。结论目前动物感染小肠结肠炎菌普遍,猪感染最高,局部已爆发流行,假结核菌偏低,鼠疫处于静息期。

Indentify of 65 strains of Yersinia and study on character of plague focuses in Guangdong

Objectives To identify 65 strains of Yersinia,to investigate biological characteristics of these Yersinia,and the relativity between Y.pestis and antigen and antibody to research the status quo and nature of plague focus.Methods Serum agglutination assay with diagonal serum of 3 kinds of Yersinia,biochemical tests,biological features were respectively performed with 65 strains Yersinia.Virulence factors,virulence gene,pathogenicity,cross-immunity,animal tests,serological tests and drug sensitivity tests were respectively conducted with 14 strains Yersinia.Results There are 63 strains of Y.enterocolitica(42 strains are O∶3 serological type and 21 strains are O∶9 serological type),one strain was Y.intermedia and one strain was Y.pseudotubercolosis.2 strains were vw+,10 strains were vw±,2 strains were vw-.F1Ag and F1Ab of 14 strains were negative.There were two virulence gene(ail,ystA) exist in 5 strains of Y.enterocolitica,vw+ and vw± strains were pathogenic to white mouse and rabbit.Cross immunity existed in animals when were infected by Yersinia.Cross reaction was nonexistent between 3 kinds of Yersinia(Y.enterocolitica,Y.pseudotubercolosis,Y.intermedia) and Y.pestis(IHA,RIHA).These Yersiniawere sensitive to common antibiotics.Conclusions The Y.enterocolitica-carrying rate of animal in Guangdong is universal,the Y.enterocolitica-carrying rate of pig is the highest even breaks out in some areas.The Y.pseudotubercolosis-carrying rate of animal is low.Plague focus of Guangdong is in resting stage.