沙苑子提取物对丝裂霉素C致小鼠骨髓细胞遗传损伤的保护作用

目的:探讨沙苑子提取物(Flastem milkvetch seed extract,FMSE)对丝裂霉素C(mitomycin C,MMC)诱导的小鼠骨髓细胞遗传损伤的保护作用。方法:将100只昆明小鼠随机分成5组,每组20只。试验分为正常对照组(生理盐水),模型组(MMC,2.0mg/kg),FMSE低(20mg/kg、中(40mg/kg)、高(80mg/kg)剂量组。灌胃给药,连续10 d,每天1次。末次灌胃前12 h,除正常对照组腹腔注射等体积生理盐水外,其余各组均腹腔注射MMC(2.0 mg/kg)。用药结束后,脱颈椎处死小鼠,制备小鼠骨髓细胞悬液,应用彗星电泳、嗜多染红细胞微核形成、骨髓淋巴细胞染色体畸变和有丝分裂指数试验,检测FMSE对MMC所诱导的小鼠骨髓细胞遗传损伤的保护作用。结果:与正常对照组比较,模型组中MMC单独作用时可造成小鼠骨髓淋巴细胞拖尾率及平均尾长、微核率和染色体畸变率显著增加(P均<0.01),有丝分裂指数显著降低(P<0.01)。而FMSE低、中、高剂量组与MMC联合作用时,与模型组比较,小鼠骨髓淋巴细胞拖尾率及平均尾长、微核率和染色体畸变率显著降低(P均<0.01),有丝分裂指数显著提高(P<0.01),。结论:FMSE对MMC诱导的小鼠骨髓细胞遗传损伤有明显的保护作用。

The protective action of the Flastem milkvetch seed extract on the genetic damage in bone marrow lymphocytes induced by mitomycin C in mice

OBJECTIVE： To study the protection of Flastem milkvetch seed extract （FMSE） on the genetic damage of bone marrow lymphocyte induced by mitomycin C （MMC） in mice. METHODS： 100 Kunming mice were divided into 5 groups at random, normal saline in control group and 2 mg/kg MMC were taken model groups. FMSE 20, 40 and 80 mg/（kg . d）were given daily in the low, medium and high dose groups for 10 d. 12 hours before the last gastric gavage, the mice received intraperitoneal MMC （2 mg/kg） except for normal group which received NS. Then the mice were killed by cervical dislocation. The bone marrow lymphocytes suspension was prepared. Comets electrophoresis, formation of micronucleus of polychromatic erythroblasts （PCE）, chromosome aberration test in bone marrow lymphocytes of mice and mitotic index （MI） were determined to study the protection of FMSE on the genetic damage of bone marrow lymphocytes induced by MMC. RESULTS： After using MMC alone in model group, rates of tailing, tail length, micronucleus and chromosome aberration of the bone marrow lymphocytes were significantly enhanced （P〈0.01） compared with those of normal group. Meanwhile MI of model group was significantly lower than that of normal group （P〈0.01）. After using different concentrations of high, medium and low FMSE combined with intrapertioneal MMC, rates of tailing, tail length, micronucleus and chromosome aberration of the bone marrow lymphocytes were significantly decreased compared with those of control group （P〈0.01）. MI of model group was dramatically higher than that of normal group （P〈0.01）. CONCLUSION： FMSE could significantly protect the genetic damage of bone marrow lymphocytes induced by MMC in mice.