| RP-HPLC法测定保康北柴胡中柴胡皂苷a、d的含量 |
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| 引用本文： | 熊梦晓,黄必胜,白杨,范文乾,胡继鹰.RP-HPLC法测定保康北柴胡中柴胡皂苷a、d的含量[J].现代中药研究与实践,2007,21(2):42-44. |
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| 作者姓名： | 熊梦晓 黄必胜 白杨 范文乾 胡继鹰 |
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| 作者单位： | 湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061 |
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| 基金项目： | 湖北省自然科学基金(2005ABA187) |
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| 摘 要： | 目的采用RP-HPLC法测定保康北柴胡中柴胡皂苷a、d的含量。方法色谱柱:BonChrom C18柱(4.6 mm×200 mm,5μm),流动相:乙腈-水(40∶60),流速:1.0 mL.min-1,检测波长:204 nm。结果柴胡皂苷a:Y=7.131 4X+2.011 8,r=0.999 7,线性范围:2.136~21.360μg;柴胡皂苷d:Y=9.032 3X+3.739 2,r=0.999 0,线性范围:2.656~26.560μg。平均回收率:柴胡皂苷a 99.3%(RSD=0.73%),柴胡皂苷d99.4%(RSD=0.82%)。结论本法简便快捷,结果准确,重复性好,GAP基地的柴胡皂苷含量较高。
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| 关 键 词： | 柴胡 柴胡皂苷 RP-HPLC |
| 文章编号： | 1673-6427（2007）02-0042-03 |
| 收稿时间： | 2007-01-08 |
| 修稿时间： | 2007-01-08 |
Determination of Saikosaponin a, d in Bupleurum by RP-HPLC |
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| XIONG Meng-xiao,HUANG Bi-sheng,HU Ji-ying.Determination of Saikosaponin a, d in Bupleurum by RP-HPLC[J].Research and Practice on Chinese Medicines,2007,21(2):42-44. |
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| Authors: | XIONG Meng-xiao HUANG Bi-sheng HU Ji-ying |
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| Abstract: | Objective A HPLC method was established for the determination of Saikosaponin a and Saikosaponin d in Bupleurum.Methods A BonChrom C18 column was used with acetonitrile-water(40∶60) as the mobile phase and in a flow rate of 1.0 mL·min-1.The detecting wavelength was at 204nm.Results There was a good linearity in the range of 2.136～21.360 μg of Saikosaponin a(r=0.999 7) and 2.656～26.560μg of Saikosaponin d(r=0.999 0),The average recovery was 99.3% and 99.4%,respectively.Conclusion The method was simple,rapid,accurate and reproducible.It has also the higher Saikosaponin content of Bupleurum chinense in GAP Base. |
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| Keywords: | Radix Bupleuri saikosaponin RP-HPLC |
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