| 致敏小鼠树突状细胞和T细胞Notch配体/受体的表达及大剂量过敏原的影响 |
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| 引用本文： | 宋云熙,曾雪峰,夏俊波,王长征.致敏小鼠树突状细胞和T细胞Notch配体/受体的表达及大剂量过敏原的影响[J].重庆医学,2007,36(10):942-944. |
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| 作者姓名： | 宋云熙 曾雪峰 夏俊波 王长征 |
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| 作者单位： | 第三军医大学新桥医院全军呼吸内科研究所,重庆,400037 |
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| 摘 要： | 目的探讨致敏小鼠Notch信号表达缺陷及大剂量过敏原对其影响。方法RT-PCR法分别检测致敏及正常小鼠树突状细胞Notch配体及T细胞Notch受体的mRNA表达情况,并观察大剂量过敏原作用对其表达的影响。结果（1）致敏小鼠树突状细胞Notch配体Jagged1,Jagged2和Delta1的mRNA表达均显著低于正常小鼠,致敏小鼠T细胞Notch1和Notch3的mRNA表达也显著低于正常小鼠（P〈0.05）。（2）10mg/ml的OVA作用下,Jagged1和Notch3的mRNA表达水平较对照组显著升高（P〈0.05）。结论致敏小鼠Notch信号表达缺陷,大剂量过敏原（10mg/ml的OVA）可上调Jagged1和Notch3表达。
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| 关 键 词： | Notch 变应性 免疫耐受 |
| 文章编号： | 1671-8348(2007)10-942-03 |
| 修稿时间： | 2006年10月2日 |
Expression of Notch in spleen DC and T cells and effects of high dose allergen in allergic mice |
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| SONG Yun-xi,ZENG Xue-feng,XIA Jun-bo,et al..Expression of Notch in spleen DC and T cells and effects of high dose allergen in allergic mice[J].Chongqing Medical Journal,2007,36(10):942-944. |
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| Authors: | SONG Yun-xi ZENG Xue-feng XIA Jun-bo |
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| Abstract: | Objective To investigate the expression of Notch in allergic mice and the effects of high dose allergen.Methods The mRNA levels of Notch ligands and receptors in spleen DC and T cells in allergic mice and normal mice were measured by RT-PCR.Spleen DC and T cells separated from allergic mice were treated with OVA at 10mg/ml or saline.And the mRNA levels of Jagged1 and Notch3 were measured by RT-PCR.Results(1)The mRNA levels of Jagged1,Jagged2 and Delta1 in DC and the mRNA levels of Notch1 and Notch3 in T cells were significantly decreased in allergic mice(P<0.05).(2)The mRNA levels of Jagged1 and Notch3 were significantly increased by OVA at 10mg/ml in spleen DC and T cells in allergic mice(P<0.05).Conclusion The expression of Notch was decreased in allergic mice,which could be increased by high dose allergen. |
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| Keywords: | Notch allergen immunotolerance |
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