基因芯片检测肠道致病菌技术的建立和应用

目的 快速、准确地检测水中存在的肠道致病菌。方法 采用基因芯片技术对水中常见肠道致病菌进行检测和鉴定，包括检测靶基冈的扩增、基因芯片的制备、杂交反应和杂交结果的检测与分析四个步骤。结果 应用基因芯片对涉及9个菌属的143株致病菌在相同的条件下进行了杂交检测，得到菌属(科)特异性的杂交图谱，从而达到对细菌进行检测鉴定的目的。结论 制备的基因芯片能够同时检测沙门菌属、志贺菌属、埃希菌属、葡萄球菌属、变形杆菌属的细菌以及小肠结肠炎耶尔森菌、副溶血性弧菌等。

Detection and identification of intestinal pathogenic bacteria by oligonucleotide array

Objective To detect the intestinal pathogenic bacteria quickly and accurately from water. Methods An experimental procedure is set up using the gene chip technology to detect and identify common intestinal pathogenic bacteria from water. Target gene was amplified and hybridized with prepared gene chip.Results 143 strains of bacteria in pure culture belong to 9 genera are successfully discriminated under comparatively same condition and a series of specific hybridization maps corresponding to each kinds of bacteria are obtained.Conclusions Salmonella spp., Shigella spp., Staphylococcus spp., Proteus spp., Escherichia coli, Yersinia enterocolitica, Listeria monocytogenes and Vibrio parahaemolyticus are detected and identified by the technology of gene chip. The accuracy, range, and discriminatory power of the assay can be continually improved by adding further oligonueleotides to the arrays without significantly increasing complexity or cost.