| 反相高效液相色谱法测定当归挥发油中藁苯内酯含量 |
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| 引用本文： | 张京,刘雪英,姜茹,罗晶,王庆伟.反相高效液相色谱法测定当归挥发油中藁苯内酯含量[J].第四军医大学学报,2009,30(9):853-855. |
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| 作者姓名： | 张京 刘雪英 姜茹 罗晶 王庆伟 |
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| 作者单位： | 张京,王庆伟,ZHANG Jing,WANG Qing-Wei(第四军医大学唐都医院药剂科,陕西,西安,710038);刘雪英,姜茹,LIU Xue-Ying,JIANG Ru(第四军医大学药学系药物化学教研室,陕西,西安,710033);罗晶,LUO Jing(陕西省食品药品检验所,陕西,西安,710061)
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| 基金项目： | 陕西省中医药管理局招标科研课题 |
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| 摘 要： | 目的：采用反相高效液相色谱法测定当归挥发油中藁苯内酯含量,为当归挥发油皮肤渗透动力学研究提供依据.方法：以乙腈-水（78∶22）为流动相,流速1.0mL/min,25℃下经Inertsil ODS-3（4.6mm×250mm,5μm）色谱柱分离,于280nm波长处检测.结果：藁苯内酯与挥发油中其它成分的分离效果良好,在0.5～80.0mg/L浓度范围内呈良好的线性关系,回归方程为：Y=45342.52X-8462.62（r=0.9998,n=5）;平均回收率为100.07%,相对标准偏差为0.18%.结论：本方法简便、准确,可快速测定当归挥发油中藁苯内酯的含量,可为质量控制提供依据.
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| 关 键 词： | 藁苯内酯 当归挥发油 反相高效液相色谱 |
Determination of ligustilide in essential oil of Angelica sinensis (Oliv.) Diels by RP-HPLC |
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| ZHANG Jing,LIU Xue-Ying,JIANG Ru,LUO Jing,WANG Qing-Wei.Determination of ligustilide in essential oil of Angelica sinensis (Oliv.) Diels by RP-HPLC[J].Journal of the Fourth Military Medical University,2009,30(9):853-855. |
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| Authors: | ZHANG Jing LIU Xue-Ying JIANG Ru LUO Jing WANG Qing-Wei |
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| Institution: | ZHANG Jing1,LIU Xue-Ying2,JIANG Ru2,LUO Jing3,WANG Qing-Wei11Department of Pharmacy,Tangdu hospital,Fourth Military Medical University,Xi'an 710038,China,2Department of Medicinal Chemistry,School of Pharmacy,Xi'an 710033,3Shaanxi Institute for Food and Drug Control,Xi'an 710061 |
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| Abstract: | AIM： To establish a reversed-phase high performance liquid chromatographic （ RP-HPLC ） method for simultaneous determination of ligustilide in the essential oil of Angelica sinensis （Oliv.） Diels. METHODS： The mobile phase was acetonitrilewater（78：22） and the flow rate was about 1 mL/min. Inertsil ODS-3（4.6 mm × 250 mm, 5 μm） column was used and the detective wavelength was 280 nm. RESULTS： The calibration curves were linear in the concentration range of 0. 5 - 80. 0 mg/L for ligustilide. The regression equation was Y = 45 342.52 X - 8462.62 （ r = 0. 9998, n = 5 ） , the average recovery was 100.07% and the relative standard deviations （RSD） was 0.18%. CONCLUSION： Our established method is simple,quick and accurate for the simultaneous separation and determination of ligustilide in the essential oil of Angelica sinensis （ Oliv. ） Diels. |
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| Keywords: | ligustilide Essential oil of Angelica sinensis (Oliv )Diels HPLC |
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