增强CT评价肝细胞癌凋亡相关蛋白表达的价值探讨

目的:探讨增强CT评价肝细胞癌(hepatocellular carcinoma,HCC)组织中凋亡相关蛋白fas,bax,bc1-2表达的价值。方法:对38例共40个经病理证实且行动、静脉双期CT增强扫描的HCC病灶进行分析,用免疫组化SP法检测癌组织中第Ⅷ因子相关抗原(FactorⅧrelated antigen,FⅧRA),fas,bax,bc1-2和bc1-x1的表达情况,并结合其病理分级将CT增强表现特征与免疫组化的结果进行对照分析。结果:fas,bax,bc1-2和bc1-x1的阳性表达率分别为20%(8/40)、25%(10/40)、27.5%(11/40)和50%(20/40),bax和bc1-2,以及bc1-2和bc1-x1之间分别具有一定的相关性(P<0.05)。bc1-x1的表达与CT显示的病灶大小、以及病理分级具有一定的关系(P<0.05),病理分级与CT显示的边界类型以及瘤内的液化坏死有关(P<0.05)。结论:fas,bax,bc1-2和bc1-x1等蛋白在HCC组织中呈现不同程度的表达,通过CT反映调亡相关蛋白表达的价值有限。

The significance of enhanced CT to evaluate the expressions of the apoptosis proteins in hepatocellular carcinoma

Objective:To study the significance of enhanced CT to evaluate the expressions of the apoptosis proteins including fas,bax,bc1-2 and bc1-x1 in hepatocellular carcinoma(HCC).Methods:Thirty-eight cases(40 lesions) of HCC verified by histopathology and examined with enhancement dual-phase CT scanning(arterial phase and portal vein phase) were included in this study.The expressions of fas,bax,bc1-2 and bc1-x1 were detected with immunohistochemical SP method.The CT enhanced features were compared with the immunohistochemical and pathological results.Results:The positive rate of fas,bax,bc1-2 and bc1-x1 was 20%(8/40),25%(10/40),27.5%(11/40)and 50%(20/40) in HCC respectively.The correlations between bax and bc1-2,bc1-2 and bc1-x1 were found(P<0.05,P<0.01 respectively).There was a significant difference of bc1-x1 positive rate in different pathological grades(P<0.05).The close relationship between the expression of bc1-x1 and the diameters of lesions on the CT imagines was found(P<0.05),and there was a significant correlation between the pathological grades and the edges of the lesions or intratumoral deliquescence and necrosis on the CT imagines(P<0.05).Conclusion:The expressions of fas,bax, bc1-2 and bc1-x1 can be detected in HCC,and the significance to evaluate the apoptosis proteins in HCC with CT is limited.