自体外周血干细胞动员时骨髓及外周血相关细胞亚群的动态变化研究

目的：探讨自体外周血干细胞动员时，恶性血液病患者的骨髓及外周血CD34^＋和T细胞亚群的动态变化特点。方法：16例拟行自体外周血干细胞移植患者，在应用化疗＋G—CSF方法动员外周血干细胞期间，以流式细胞仪测定骨髓及外周血CD34^＋和T细胞亚群动态变化情况。结果：化疗后应用G—CSF48h后，骨髓中CD34^＋细胞开始明显增加（P〈0．01），在应用G—CSF72h后，外周血中CD34^＋细胞开始明显增加（P〈0．01），96h后，两者达峰值。较未应用G—CSF时差异显著（P〈0．01）。T淋巴细胞亚群比例处于倒置状态且变化不明显（P〉0．05）。结论：恶性血液病患者在应用化疔＋G—CSF方法动员外周血干细胞时，其骨髓及外周血CD34^＋细胞逐渐增加，于96h后达高峰，T细胞亚群变化不明显。

Dynamic changes of CD34+ cell and T lymphocyte subsets in bone marrow or peripheral blood during peripheral blood stem cell mobilization

Objective:To study the dynamic changes of CD_(34)~+ cells and T lymphocyte subsets in bone marrow or(peripheral) blood during peripheral blood stem cell mobilization by chemotherapy and G-CSF injection.Methods:Flow cytometry was used to detect CD_(34)~+ cells and T lymphocyte subsets in 16 patients treated with chemotherapy and injected with G-CSF for peripheral blood stem cells mobilization.Results: CD_(34)~+ cells in the bone marrow and peripheral blood increased obviously after injecting G-CSF for 48 h and 72 h respectively.The highest(percentages) were achieved 96 h after this procedure in both of them.There were obvious difference between before and after G-CSF injection(P<0.01).CD4/CD8 ratio was inverted in both bone marrow cells and peripheral blood cells,but there were no obvious changes(P>0.05).Conclusion: CD_(34)~+ cells in bone marrow or peripheral blood increase gradually after chemotherapy and G-CSF injection,and can reach the highest level 96 h after this procedure.T lymphocyte subsets keep stationary.