WNK4基因 G1816T多态性与高血压的关联研究

目的阐明WNK4基因G1816T多态性与原发性高血压的关系。方法2001年3~10月选择中国东北高血压病高发地区辽宁省彰武县采集的259例原发性高血压患者和235名健康对照组的血样应用聚合酶链反应-限制性长度片段长度多态性分析(PCR-RFLP)方法检测G1816T多态,结果经测序验证,同时检测所有人群的血糖、血脂等生化指标。结果原发性高血压组WNK4基因G1816T多态T等位基因频率显著高于对照组(25·9%对20·2%,P=0·035);AS和SS基因型与收缩压及舒张压升高密切相关(P分别为0·041和0·032)。结论WNK4基因G1816T多态与高血压密切相关。

Association analysis between G1816T polymorphism of WNK4 gene and essential hypertension

Objective To elucidate the association of WNK4 gene G1816T polymorphism with essential hypertension.Methods Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism( PCR- RFLP)and confirmed by direct sequencing.259 unrelated patients with essential hypertension and 235 unrelated normal controls were recruited.All the subjects had routine laboratory tests.Results The frequency of the T allele of exon8 G1816T polymorphism in hypertension group is significantly higher than that in controls(25.9% versus 20.2%, P= 0.035).The AS and SS genotypes were closely associated with raised systolic and diastolic blood pressure(P=0.041,0.032 respectively).Conclusion The WNK4 gene G1816T polymorphism is associated with essential hypertension.