四妙勇安汤对人脐静脉血管内皮细胞ECV304的增殖作用

目的:以血清药理学的方法观察血四妙勇安汤对于培养人脐静脉内皮细胞ECV304增殖的影响,为治疗性血管新生的靶基因选择提供线索。方法:取对数生长期的内皮细胞,同步化于G0期,ELISA法检测BrdU的掺入;用流式细胞仪分析细胞周期各时相分布。结果:与空白血清对照组相比,10%的四妙勇安汤含药血清体积添加分数可显著提高BrdU的掺入量,促进DNA合成,提高ECV304细胞周期S期所占比例。结论:10%的四妙勇安汤含药血清能促进内皮细胞ECV304DNA合成,加快细胞周期进程,从而对其增殖具有促进的作用,可能具有血管生成的作用。

Effect of Simiao Yongan Decoction on the Proliferation of Human Umbilical Vein Endothelial Cells

Objective: We examine the effect of Simiao Yongan decoction(SMYA) on human vascular endothelial cells by serum pharmacology and provide the evidence for "remedial angiogenesis".Methods:The proliferation effect of Simiao Yongan decoction was observed by BrdU-ELISA, flow cytometer was used to analysis the cell circles RT-PCR was used to analysis the expression changing of cyclin D.Results:compared with control group,10% serum contained SMYA group can simulate the proliferation of ECV304.The percentage of cells of drug group in S phase was increased and the expression of cyclin D was significantly raised.Conclusions:10% serum contained SMYA have the significant proliferation effect on the ECV304 and may be used as a therapeutic method of "remedial angiogenesis".