| sDR5封闭TRAIL减少内毒素引起小鼠肝细胞凋亡 |
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| 引用本文： | 白慧玲,王雪银,李淑莲,黄红莹,杜耀武,刘广超,马远方.sDR5封闭TRAIL减少内毒素引起小鼠肝细胞凋亡[J].中华微生物学和免疫学杂志,2009,29(2). |
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| 作者姓名： | 白慧玲 王雪银 李淑莲 黄红莹 杜耀武 刘广超 马远方 |
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| 作者单位： | 1. 河南大学医学院,河南大学免疫学研究所,河南省天然药物与免疫工程重点实验室,开封,475004
2. 河南职工医学院医学技术系微生物学与免疫学教研室,郑州,450002 |
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| 基金项目： | 河南省教育厅自然科学基金，河南大学自然科学基金 |
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| 摘 要： | 目的 探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)在内毒素诱导小鼠肝细胞凋亡中的作用.方法 内毒素作用于可溶性死亡受体-5(sDR5)封闭TRAIL的小鼠,不同时间检测小鼠血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)和乳酸脱氧酶(LDH)的含量;HE染色及Annexin V、PI双染法流式细胞术检测小鼠肝细胞凋亡;免疫组织化学法及流式细胞术检测小鼠肝细胞和肝细胞膜表面DR5的表达.结果 内毒素可以上调肝细胞DR5的表达.DR5封闭TRAIL可显著抑制内毒素引起的小鼠肝细胞凋亡和改善肝细胞损伤.结论 内毒素引起疾病过程中TRAIL起重要作用.
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| 关 键 词： | 内毒素 脂多糖 可溶性死亡受体5 肿瘤坏死因子相关凋亡诱导配体 肝细胞凋亡 |
Reduced apoptosis induced by endotoxin in mice blocking TRAIL with soluble death receptor 5 |
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| BAI Hui-ling,WANG Xue-yin,LI Shu-lian,HUANG Hong-ying,DU Yao-wu,LIU Guang-chao,MA Yuan-fang.Reduced apoptosis induced by endotoxin in mice blocking TRAIL with soluble death receptor 5[J].Chinese Journal of Microbiology and Immunology,2009,29(2). |
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| Authors: | BAI Hui-ling WANG Xue-yin LI Shu-lian HUANG Hong-ying DU Yao-wu LIU Guang-chao MA Yuan-fang |
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| Abstract: | Objective To explore the effect of TNF related apoptosis inducing ligand (TRAIL) in apoptosis induced by LPS. Methods After LPS injected mice blocking TRAIL with soluble death receptor 5 (sDRS), detecting ALT, AST and LDH of mice serum at different times, apoptotic effects of LPS to mice hepatocyte were detected by HE and flow eytometry (FCM) with Annexin V-FITC/PI staining. The expres-sion of DR5 in mice hepatocyte was assayed with immunohistochemistry and FCM. Results Apoptotic effect was promoted by up-regulated DR5 expression on hepatocyte. Blocking TRAIL with sDR5 markedly amelio-rated the hepatocyte damage and reduced apoptosis. Conclusion These results establish a critical role for TRAIL in apoptosis during disease process of LPS. |
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| Keywords: | Endotoxin Lipopolysaccharides Soluble death receptor 5 TNF related apoptosis inducing ligand Hepatocyte apoptosis |
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