尼莫地平对惊厥持续状态幼年大鼠海马GRP78、CHOP表达及细胞凋亡的影响

目的观察尼莫地平对惊厥持续状态（statusconvulsion，SC）幼年大鼠海马葡萄糖调节蛋白78／免疫球蛋白重链结合蛋白Bip（GRP78／Bip）、前凋亡因子（CHOP／GADD153）表达及神经元凋亡的影响。方法雄性幼年SD大鼠117只，随机分为正常对照组（NC组）、惊厥持续状态组（SC组）、尼莫地平预处理组（NM组）三大组；分别予生理盐水、氯化锂-匹罗卡品、尼莫地平和氯化锂-匹罗卡品进行处理，各组又均随机分为3个亚组，分别于4、24、48h处死。采用氯化锂-匹罗卡品法制作SC模型；RT—PCR技术检测海马GRP78／Bip和CHOP／GADD153mRNA表达的动态变化。原位末端标记（TUNEL）检测海马CA1区中神经细胞的凋亡。结果SC组各时间点海马CA1区TUNEL阳性细胞表达均明显高于NC组（均P〈005或001oNM组各时间点TUNEL阳性细胞较SC组低，但高于NC组（P〈0．05或0．01）。与NC组相比，SC组各时间点海马GRP78／BipmRNA和CHoP／GADD153mRNA表达均升高。NM组GRP78／BipmRNA、CHOP／GADD153mRNA表达情况与SC组表达规律类似，但表达水平有差别。结论NM预处理可以影响CHOP／GADD153和GRP78／Bip的表达，缓解内质网应激，减轻惊厥后海马神经元细胞凋亡程度。

Effect of nimodiping on expression of GRP78/Bip and CHOP/GADD153 mRNA in hippocampus of rats with status convulsion

Objective To investigate the effect of nimodiping on the expression of GRP78/Bip and CHOP/GADD153 mRNA and TUNEL-positive cells in the hippocampus of rats with status convulsion. Methods One hundred and seventeen male juvenile Sprague-Dawley （SD） rats were randomly divided into normal control group （ NC ）, status convulsion group （SC）and NMravone group （NM）. Status convulsion （SC）was induced by injecting lithium chloride and pilocarpine,the expression of GRP78/Bip and CHOP/GADD153 mRNA were detected by RT-PCR,the neural apoptosis in CA1 domain on the hippocampus were detected by TUNEL . Results Compared to group NC TUNEL-positive cells in CA1 domain on the hippocampus were increased in group SC; while TUNEL positive cells in group NM were lower than those in group SC but higher than group NC. The expression of GRP78/Bip and CHOP/GADD153 mRNA in the group SC was much higher than that in group NC,there was no difference between group NM and group SC. Conclusion NM can affect the expression of GRP78/Bip and CHOP/GADD153, relieve ERS and lessen the pathology of neural apoptosis in CA1 domain on the hippocampus.