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| 淋病奈瑟菌毒力岛基因的检测 | |
| 引用本文： | 崔海燕,李国明,陈群,樊翌明. 淋病奈瑟菌毒力岛基因的检测[J]. 中国皮肤性病学杂志, 2011, 25(2): 104-106 |
| 作者姓名： | 崔海燕 李国明 陈群 樊翌明 |
| 作者单位： | 1. 深圳市龙岗区人民医院检验科,广东,深圳,518172 2. 广东医学院微生物学免疫学教研室,广东,湛江,524023 3. 广东医学院附属医院皮肤性病研究所,广东,湛江,524001 |
| 基金项目： | 广东省自然科学基金资助 |
| 摘 要： | 目的 了解淋病奈瑟菌毒力岛(PAIs)在质粒及染色体上的分布情况.方法 采用碱裂解法提取63株淋病奈瑟菌的质粒及染色体DNA,PCR扩增各PAIs基因(atlA,traG,traH).结果 共检出4.2kb,7.4kb,39.5kb,42.5kb四种质粒,总检出率为88.89%(56/63);以63株淋病奈瑟菌的染色体... |
| 关 键 词： | 淋病奈瑟菌 质粒 毒力岛 |
Detection the Pathogenicity Islands Genes of Neisseria Ggonorrhoeae |
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| CUI Hai-yan,LI Guo-ming,CHEN Qun,FAN Yi-ming. Detection the Pathogenicity Islands Genes of Neisseria Ggonorrhoeae[J]. The Chinese Journal of Dermatovenereology, 2011, 25(2): 104-106 | |
| Authors: | CUI Hai-yan LI Guo-ming CHEN Qun FAN Yi-ming |
| Abstract: | Objective To explore the distribution of the gonococcal pathogenicity islands(PAIs) on chromosome and plasmid. Methods The plasmids and chromosome of 63 Neisseria gonorrhoeae strains were separately extracted by alkaline lysis technique. PAIs genes atlA,traG and traH were amplified by PCR and located. Results Of the 63 Neisseria gonorrhoeae strains,the plasmids of 4.2kb,7.4kb,39.5kb and 42.5kb were detected. The PCR results of PAIs genes in chromosome DNA showed that the detection rates of atlA,traG and traH were 74.60%(47/63),34.92%(22/63),68.25%(43/63),respectively. By using the same method,the detection rates of atlA,traG and traH in plasmid DNA were 75.00%(42/56),62.50%(35/56) and 64.29%(36/56),respectively. Conclusion The gonococcal pathogenicity islands generally present not only in chromosome,but also in plasmid. Its detection rate is very high. |
| Keywords: | Neisseria gonorrhoeae Plasmid Pathogenicity islands |
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