白癜风患者血浆中氧化与抗氧化相关指标的检测

目的通过对白癜风患者及健康对照者血浆中氧化与抗氧化相关指标——超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、维生素E(VitE)及一氧化氮(NO)表达水平的测定,探讨氧化应激在白癜风发病机制及疾病发展中的作用和意义。方法选择60例白癜风患者(患者组)和40名健康志愿者(健康对照组)为研究对象,化学法检测血浆中SOD,CAT,GSH-Px的活性及MDA,VE和NO的含量。结果患者组血浆中MDA含量及SOD活性明显高于健康对照组(P<0.01),而GSH-Px活性明显低于健康对照组(P<0.01);进展期白癜风患者血浆中MDA的含量及SOD的活性明显高于稳定期白癜风组(P<0.01),而GSH-Px活性明显低于稳定期白癜风患者(P<0.01);随MDA含量增加,GSH-Px活性逐渐降低,呈显著负相关关系(r=-0.337,P<0.01),而SOD活性逐渐升高,呈显著正相关关系(r=0.347,P<0.01);而在进展期和稳定期白癜风患者血浆中CAT,VE和NO的含量与健康对照组相比差异无统计学意义(P>0.05)。结论白癜风患者血浆中存在氧化-抗氧化失衡,白癜风的发病及病情活动与氧化应激可能相关。

Assay of Oxidative Stress in Plasma of Patients with Vitiligo

Objective To evaluate the oxidative stress in the pathogenesis of vitiligo by examining indicators of oxidative stress in plasma,which include superoxide of dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),malonaldehyde(MDA),VitE(Vitamin E)and NO(nitric oxide).Methods Sixty patients with vitiligo and 40 healthy volunteers were included in the study.Plasma levels of SOD,CAT,GSH-Px,MDA,VitE and NO were determined by chemical method.Results Our results revealed a significantly higher level of plasma MDA and SOD,a lower level of GSH-Px in 60 patients with vitiligo compared with the control(P<0.01);Compared with the control,the MDA levels and SOD activities in plasma in the active vitiligo group were significantly increased(P<0.01),whlile the GSH-Px activities,levels in the active vitiligo group were significantly decreased(r=-0.337,P<0.01);With the increase of the MDA,there was a linear decrease of activities about GSH-Px in plasma and a linear increase of acitivities about SOD in plasma(r=0.347,P<0.01).Compared with the control,the CAT,VE and NO levels in plasma in the active and stable vitiligo groups were not increased significantly(P>0.05).Conclusion Our study shows that oxidative stress is involved in vitiligo,and that oxidative stress is associated with the activity of vitiligo.