| 姜黄素对IL-17诱导的人表皮角质形成细胞株CCL20表达的影响 |
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| 引用本文： | 杨正生,彭振辉,李晓莉,宋健文,任建文,尧志建. 姜黄素对IL-17诱导的人表皮角质形成细胞株CCL20表达的影响[J]. 中国皮肤性病学杂志, 2011, 25(5) |
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| 作者姓名： | 杨正生 彭振辉 李晓莉 宋健文 任建文 尧志建 |
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| 作者单位： | 1. 西安交通大学医学院第二附属医院皮肤科,陕西,西安,710004;河北省秦皇岛市第一医院,河北,秦皇岛,066000
2. 西安交通大学医学院第二附属医院皮肤科,陕西,西安,710004 |
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| 摘 要： | 目的探讨姜黄素对IL-17诱导的人表皮角质形成细胞株(HaCaT细胞株)CCL20表达的影响。方法用IL-17刺激HaCaT细胞,并加入不同浓度的姜黄素(2.5μmol/L,5μmol/L,10μmol/L)共培养24h。提取总RNA并收集细胞上清液,分别进行荧光定量PCR和ELISA实验,从mRNA及蛋白表达水平两方面观察姜黄素对CCL20表达的影响。结果 IL-17能够有效地诱导HaCaT细胞CCL20的表达。加入姜黄素共培养后,CCL20在mRNA及蛋白表达水平均明显下降(P均<0.05)。结论姜黄素对IL-17诱导的HaCaT细胞CCL20的表达具有明显的抑制作用,可为其对银屑病的治疗提供理论依据。
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| 关 键 词： | 姜黄素 CCL20 白介素-17 角质形成细胞 |
Effect of Curcumin on IL-17-Induced CCL20 Expression in Human Keratinocytes |
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| YANG Zheng-sheng,PENG Zhen-hui,LI Xiao-li,SONG Jian-wen,REN Jian-wen,YAO Zhi-jian. Effect of Curcumin on IL-17-Induced CCL20 Expression in Human Keratinocytes[J]. The Chinese Journal of Dermatovenereology, 2011, 25(5) |
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| Authors: | YANG Zheng-sheng PENG Zhen-hui LI Xiao-li SONG Jian-wen REN Jian-wen YAO Zhi-jian |
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| Affiliation: | YANG Zheng-sheng1,2,PENG Zhen-hui1,LI Xiao-li1,SONG Jian-wen1,REN Jian-wen1,YAO Zhi-jian1(1.Department of Dermatology,the Second Affiliated Hopital of Xi'an Jiaotong University,Xi'an 710004,China,2.Department of Dermatology,the First Hospital of Qinhuangdao,Qinhuangdao 066000,China) |
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| Abstract: | Objective To explore the effect of curcumin on IL-17-induced CCL20 expression in human keratinocyte cell lines,HaCaT cells.Methods HaCaT cells were stimulated with IL-17 and incubated with different concentrations of curcumin(2.5μmol/L,5μmol/L,10μmol/L) for 24h.To determine the effect of curcumin on CCL20 expression at mRNA and protein levels,fluorescence quantitative PCR and ELISA were performed.Results IL-17 led to the upregulation of CCL20 expression in HaCaT cells.After incubation with curcumin,the expression of CCL20 were down-regulated at mRNA(P<0.05) and protein(P<0.05) levels significantly.Conclusion Curcumin can lead to the downregulation of IL-17-induced CCL20 expression in HaCaT cells,thus provides a theoretical basis for the treatment of psoriasis. |
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| Keywords: | CCL20 |
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