大鼠局灶性脑缺血模型制备方法的比较

目的比较线栓法、电凝大脑中动脉同时结扎单侧颈总动脉（简称电凝单侧结扎）和电凝大脑中动脉同时结扎双侧颈总动脉（简称电凝双侧结扎）3种方法制作局灶性大鼠脑缺血模型的优劣。方法按照动物模型制备方法的不同，将20只大鼠随机分为线栓组（6只）、电凝单侧结扎组（7只）和电凝双侧结扎组（7只）。采用三种方法制备大鼠局灶性脑缺血模型，用激光多普勒血流仪测得梗死前后的脑血流量的相对值，24h后对模型进行神经功能缺损评分，行2，3，5-氯化三苯基四氮唑染色，计算脑梗死体积。结果①线栓组（9．6±0．6）和电凝双侧结扎组（9．8±0．6）大鼠的神经功能缺损评分高于电凝单侧结扎组（5．7±0．7），差异有统计学意义，P〈0．01；②线栓组（132±16）mm3和电凝双侧结扎组（142±20）mm3大鼠的梗死体积高于电凝单侧结扎组（40±11）mm3，差异有统计学意义，P〈0．01；③电凝双侧结扎组的脑血流量相对值低于电凝单侧结扎组，差异有统计学意义，P〈0．05；再灌注后，线栓组的脑血流量相对值高于电凝双侧结扎组，差异有统计学意义，P〈0．05。结论三种方法建立大鼠局灶性脑缺血模型，各有优缺点，应根据研究目的采用不同的模型。

Comparison of the establishment methods of focal cerebral ischemic model in rats

Objective To compare the advantages and disadvantages of the intraluminal suture, electrocautarization of middle cerebral artery combined with unilateral ligation of common carotid artery and electrocautarization of middle cerebral artery combined with bilateral ligation of common carotid artery for establishment of focal cerebral ischemia models in rats. Methods The focal cerebral isehemia rat models were induced by 3 means. Twenty rats were randomly allocated into intraluminal suture group （ n = 6）, electrocautarization with unilateral ligation group （ n = 7 ） , and eleetroeautarization with bilateral ligation group （ n = 7 ） according to the different methods for establishment of the rat model. A laser Doppler flow metry was used to detect the relative values of the cerebral blood flow before and after cerebral infarction. The neurological deficit scores of models were measured after 24 h, and the brain slices were stained with 2, 3, 5-triphenyl tetrazolium chloride. The volume of cerebral infarction was calculated. Results （1）The neurological deficit scores in the intraluminal suture group （9.6 ±0. 6） and the electrocautarization with bilateral ligation group （9.8 ±0. 6） were higher than those in the eleetroeautarization with unilateral ligation group （5.7±0. 7）. The differences were statistically significant （P 〈 0. 01 ）. （2）The volume of cerebral infarction in the intraluminal suture group （ 131.5 ±15.8 ） mm3 and the electrocautarization with bilateral ligation group （ 141.7 ±19. 8 ）mm3 were larger than that in the electroeautarization with unilateral ligation group （39. 9 ± 11.4）mm3. The differences were statistically significant （P 〈 0.01 ）. （3）The relative value of cerebral blood flow （CBF） the eleetrocautarization with in the electrocautariza on with bilateral ligation group was lower than that in unilateral ligation group. The difference was statistically significant （P 〈0. 05）. After reperfusion, the relative value of CBF in the intraluminal suture group was higher than that in the electrocautarization with bilateral ligation group. The difference was statistically significant （P 〈 0. 05）. Conclusion The three methods had their advantages and disadvantages in inducing iocal cerebral ischemia model in rats. A difterent model should be used according to the purposes of research.