酸碱对药大黄与黄柏在大黄硝石汤中配伍的化学研究

目的：考察酸碱对药大黄与黄柏在大黄硝石汤中配伍后酸、碱性成分的变化情况。方法：采用高效液相色谱法对大黄、黄柏单味药提取液、大黄黄柏对药提取液及全方提取液中蒽醌类成分和小檗碱的提取量进行了测定。结果：蒽醌类成分及小檗碱的提取量随着单药、对药及全方的配伍变化，水煎煮降低趋势明显，乙醇提取变化不明显，且提取量不及水提取。水煎煮单药与对药之间变化趋势尤其明显，而小檗碱的变化趋势较蒽醌类成分更明显。结论：大黄、黄柏单煎和乙醇回流、大黄、黄柏对药煎煮和乙醇回流、大黄硝石汤全方煎煮和乙醇回流，酸、碱性成分的提取量发生有规律的变化。

Compatability chemistry of acid-alkaline pair medicine of Dahuang and Huangbai in Dahuangxiaoshi decoction

Objective: To determine the change pattern of alkaline and acid components of dahuang and huangbai amoung different combinations in Dahuangxiaoshi decotion. Method: The contents of anthraquinones and berberine were determined by HPLC in samples of Dahuang extracts, Huangbai extracts, Dahuang and Huangbai pair medicines extracts, and extracts of whole recipe. Result: The contents of anthraquinones and berberine were decreased with the changes of combinations: from component medicinal material alone to pair medicines, and whole recipe. the change pattern was more apparent in water decoctions than in ethanol extracts. Conclusion: The contents of alkaline and acid components were changed with different extract methods and different combinations.