HPLC测定肾益康胶囊中大黄酚和大黄素的含量

目的:建立测定肾益康胶囊中大黄酚和大黄素含量的高效液相色谱法. 方法:色谱柱为SB-C18柱(150mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸溶液(85:15),流速1.0mL/min,检测波长254nm,柱温30℃.结果:大黄酚在1.996~19.96μg(r=0.9995)范围内与峰面积线性关系良好,平均回收率为98.20%,RSD为1.63%(n=6). 大黄素在4.18~41.8μg(r=0.9996)范围内与峰面积线性关系良好,平均回收率为98.25%,RSD为1.78%(n=6). 结论:该方法简便、快速、准确,可较好控制该制剂的质量.

Determination of Chrysophanol and Emodin in Shenyikang Capsules by HPLC

Objective:To establish the HPLC method for determining the content of chrysophanol and emodin in Shenyikang Capsules. Methods:The HPLC method was used with the SB-C18 column (150mm×4.6m,5μm), The mobile phase consisted of methanol-0.1%phosphoric acid solution(85:15), The flow rate was 1.0mL/min, the detection wavelength was 254 nm, and the column temperature was 30℃. Results:The calibration curve of chrysophanol was linear in the range of 1.996~19.96μg(r=0.9995) .The average recovery rate was 98.20%,RSD=1.63%(n=6). The calibration curve of emodin was linear in the range of 4.18~4.18μg (r=0.9996).The average recovery rate was 98.25%, RSD =1.78% (n=6). Conclusion:The method is simple, rapid and accurate, And it can control the quality of the preparation. effectively.