自发性高血压大鼠肾脏血管紧张素转换酶2的表达

目的  观察不同月龄的自发性高血压大鼠(SHR)的肾脏血管紧张素转换酶2(ACE2)的mRNA和蛋白质表达水平，探讨ACE2与血压状态的内在联系。  方法  12周龄雄性自发性高血压大鼠(SHR)18只和12周龄Wistar-Kyoto(WKY)大鼠18只。随机分为SHR组和WKY组，每组抽取各9只处死后与喂养12周后处死的24周龄大鼠一并进行ACE2的检测。采用实时定量RT-PCR法检测ACE2 mRNA的表达，免疫组织化学检测ACE2蛋白的表达。  结果  与同周龄WKY组比较，SHR组大鼠的血压显著增加(P均＜0.001)，ACE2 mRNA表达显著降低(P均＜0.001)；与12周龄SHR组比较，24周龄SHR的血压显著增加(P＜0.01)，ACE2 mRNA表达显著降低(P＜0.01)；与同周龄的WKY组比较，SHR组肾脏ACE2免疫染色表达显著减少。 结论  肾脏ACE2 mRNA和蛋白质的表达与血压升高相关。

Expressions of angiotensin converting enzyme 2 mRNA and protein in the kidneys in spontaneously hypertensive rats

Objective To investigate the relationship of ACE2 and blood pressure on expression levels of mRNA and protein in spontaneously hypertensive rats(SHR).Methods 18 male(12 weeks old) SHRs and 18 male(12 weeks old) Wistar Kyoto(WKY) rats were randomly divided into two groups: the WKY-control group(n=18) and the SHR group(n=18).Nine SHRs and nine WKY rats were randomly drawn for detection.After 12 weeks feeding,other rats were executed for detection.The mRNA expression of ACE2 was determined by real-time quantit...