山莨菪碱对大鼠体内梭曼解毒的影响

通过测定大鼠血液中梭曼残留浓度及组织中羧酸酯酶(CaE)活性，评价了山莨菪碱对大鼠血液中梭曼消除的影响. 结果表明，415 μg·kg^-1梭曼(5 LD₅₀)iv后15 s时，山莨菪碱即显著加速了梭曼在大鼠血液中的消除，使血中梭曼浓度由(434±70)μg·L^-1下降到(342±47)μg·L^-1；CaE活性测定结果表明，山莨菪碱有加强梭曼抑制肠道及血浆中CaE的作用，提示山莨菪碱加速了梭曼在大鼠血液中的消除，可能与该药的改善外周微循环作用有关，使更多的梭曼向外周组织中分布，并增加了梭曼与外周组织中解毒酶特别是肠道CaE的结合所致.

Effect of anisodamine on the detoxification of soman in rats

The effect of anisodamine on soman clearance from blood in rats was studied by measuring initial levels of soman in blood using electrical eel acetylcholinesterase-based microassay and by the determination of carboxylesterase activity. The results showed that anisodamine could markedly promote the disappearance of soman from blood in rats, 15 s after soman administration(415 μg·kg^-1, 5 LD₅₀, iv), soman concentrations were (434±70) μg·L^-1 blood and (342±47) μg·L^-1 blood in control rats and rats pretreated with anisodamine, respectively(P＜0.05). Pretreatment with anisodamine made the carboxylesterase inhibition in intestine and plasma more pronounced. All these results implied that anisodamine-induced decrease of soman concentration in blood might be partly due to its improvement of peripheral microcirculation, which subsequently increased the blood perfusion through the body and thereby increased the binding between soman and detoxifying enzymes in peripheral tissues.