| 高纯度积雪草总苷的制备工艺研究 |
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| 引用本文： | 陈 俊,张小龙,杨 时,原少伟,丁 岗,王振中,萧 伟. 高纯度积雪草总苷的制备工艺研究[J]. 中草药, 2014, 45(4): 504-508 |
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| 作者姓名： | 陈 俊 张小龙 杨 时 原少伟 丁 岗 王振中 萧 伟 |
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| 作者单位： | 1.江苏康缘药业股份有限公司，江苏 连云港 222001 2.中药制药过程新技术国家重点实验室，江苏 连云港 222001 3.江苏省企业院士工作站，江苏 连云港 222001 |
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| 基金项目： | “十一五”国家科技支撑计划（2006BAI11B03） |
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| 摘 要： | 目的 研究高纯度积雪草总苷的制备工艺。方法 采用大孔树脂分离技术与重结晶技术相结合的方法，优选最佳积雪草总苷制备工艺参数，同时建立积雪草总苷HPLC检测方法。结果 积雪草药材采用75%乙醇回流提取、回收乙醇、水沉、酸沉，通过HPD-100大孔树脂柱富集后，再采用醋酸乙酯重结晶方法，可制备质量分数90%以上的积雪草总苷。结论 该工艺稳定性高、重复性好，适合于制备高纯度的积雪草总苷。
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| 关 键 词： | 积雪草；积雪草总苷；积雪草苷；羟基积雪草苷；积雪草苷B；HPLC |
Preparing technology of asiaticosides with high-purity |
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| CHEN Jun,ZHANG Xiao-long,YANG Shi,YUAN Shao-wei,DING Gang,WANG Zhen-zhong,XIAO Wei. Preparing technology of asiaticosides with high-purity[J]. Chinese Traditional and Herbal Drugs, 2014, 45(4): 504-508 |
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| Authors: | CHEN Jun ZHANG Xiao-long YANG Shi YUAN Shao-wei DING Gang WANG Zhen-zhong XIAO Wei |
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| Affiliation: | 1.Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China 2.State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China 3.Jiangsu Enterprise Academician Workstation, Lianyungang 222001, China |
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| Abstract: | Objective To study the preparing technology of asiaticosides with high purity. Methods Macroreticular resin column chromatography and recrystallization were used to optimize the preparing technology of asiaticosides. HPLC method was established for the contcent determination of asiaticosides. Results Centella asiatica was subjected to 75% ethanol distilment, water and acid precipitation, HPD100 macroreticular resin separation, and recrystallization in acetic ester. Then asiaticosides with high purity over 90% were obtained. Conclusion The process is stable and feasible, and it could be used for the preparation of high-purity asiaticosides. |
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| Keywords: | Centella asiatica (L.) Urban asiaticosides asiaticoside madecassoside asiaticoside B HPLC |
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