重楼皂苷Ⅰ通过PI3K/Akt途径诱导胰腺癌PANC-1细胞凋亡的研究

目的]评价重楼皂甙I对胰腺癌PANC-1细胞增殖和凋亡的影响。方法]以体外培养的胰腺癌PANC-1细胞为研究对象。MTr法检测重楼皂甙I对PANC．1细胞增殖的抑制作用，Annexin．V—FITC／PI双染法检测细胞凋亡，WesternBlot法检测P13K、Akt、pAkt、Bax、Bcl-2、caspase-3蛋白表达改变。结果]不同浓度重楼皂甙I能有效抑制PANC-1细胞的增殖，且呈时间浓度依赖性（P〈0．01）。低、中、高浓度重楼皂甙I作用PANC-1细胞24、d8h后，细胞凋亡率明显增加，与对照组相比，具有统计学差异（P〈0．01）。2μg／ml重楼皂甙I作用PANC．1细胞48h后，P13K、pAkt、Bcl．2蛋白表达降低．caspase-3、Bax蛋白表达增加，与对照组相比，具有统计学差异（P〈0．01）。结论]重楼皂甙I能抑制胰腺癌PANC．1细胞的体外增殖，诱导细胞凋亡，机制可能与降低P13K、pAkt、Bcl-2蛋白表达，增加Bax及caspase-3蛋白表达有关。

The Effect of Paris Saponin I on Apoptosis Associating with PI3K/Akt Pathway in Pancreatic Carcinoma Cell Line PANC-1

Purpose] To investigate the effect of Paris Saponin I on proliferation and apoptosis in pancreatic carcinoma cell line PANC-1. Methods] The effects of Paris Saponin I on cell proliferation and cell apoptosis were detected by MTY method and Annexin-V-FITC/PI respec- tively. The expressions of PI3K,Akt,pAkt,Bax,Bcl-2 and caspase-3 were detected by Western Blot.Results] The growth of PANC-1 cells was inhibited by Paris Saponin I in dose-depen- dent and time-dependent manner. Cell apoptosis was induced significantly after treated with dif- ferent concentration of Paris Saponin I at 24h and 48h（P〈0.01）. The expression of PI3K,pAkt and Bcl-2 protein decreased,while the expressions of Bax and caspase-3 protein increased after treated with 2μg/ml Paris Saponin I at 48h. Conclusion] Paris Saponin I can inhibit cell proliferation of pancreatic carcinoma cell line PANC-1 in dose-dependent and time-dependent manner and induce cell apoptosis,associating with decreasing expression of PI3K,pAkt and Bcl-2,increasing expression of Bax and easpase-3.