HPLC法同时测定丹灯通脑胶囊中野黄芩苷和丹酚酸B的含量

目的建立丹灯通脑胶囊中野黄芩苷和丹酚酸B含量的测定方法。方法采用HPLC法,色谱柱为Diamonsil C18(200 mm×4.6 mm,5μm),流动相为体积分数为2%的冰醋酸水溶液(溶液A)和乙腈-甲醇(体积比为1:1,溶液B),进行梯度洗脱,在0~15 min,溶液B体积分数由30%线性改变至36%;15~25 min,维持溶液B体积分数为36%不变;25~30 min,溶液B体积分数由36%线性改变至30%,流速为1.0 mL.min-1,检测波长为280 nm,柱温为35℃。结果野黄芩苷和丹酚酸B分别在5.7~57.0 mg.L-1和22.2~222.0 mg.L-1内与色谱峰面积线性关系良好,相关系数r分别为0.9998和0.9999。野黄芩苷和丹酚酸B的平均回收率分别为99.7%和100.3%,RSD(n=9)分别为0.9%和0.5%。结论HPLC法简便、专属、重复性好,可用于丹灯通脑胶囊中野黄芩苷和丹酚酸B的含量测定。

Simultaneous determination of scutellarin and salvianolic acid B in Dandengtongnao capsules by HPLC

Objective To develop a method for determination of scutellarin and salvianolic acid B in Dandengtongnao(traditional Chinese medicines) capsules by HPLC.Methods The analysis was carried on a Diamonsil C18(200 mm × 4.6 mm,5 μm,Dikma)column.The mobile phase was composed of 5% acetic acid(A) and acetonitrile-methanol(1∶1) with gradient elution(0-15 min,30%-36% B,15-25 min,36% B,25-30 min,30%-36% B,volumn fraction),the flow rate was 1.0 mL·min-1.The detection wavelength was set at 280 nm and column temperature was 35 ℃.Results The linear range was 5.7-57.0 mg·L-1(0.999 8) for scutellarin,22.2-222.0 mg·L-1(0.999 9) for salvianolic acid B.The average recoveries were 99.7%(n=9,RSD=0.9%) for scutellarin and 100.3%(n=9,RSD=0.5%) for salvianolic acid B.Conclusions The method is convenient,selective and reproducible for determination of scutellarin and salvianolic acid B in Dandengtongnao capsules by HPLC.