超声检测骨髓干细胞水凝胶制剂对实验兔心肌梗死后左心室功能恢复的研究

目的 应用超声检测家兔急性心肌梗死(AMI)模型骨髓干细胞水凝胶复合物注射前后心功能,评价该方法治疗心肌梗死的疗效.方法 56只兔建立左室前壁AMI模型,制备BrdU标记骨髓干细胞(BMSCs)水凝胶复合物.AMI后1周存活兔52只分为4组行梗死区心外膜注射:①处理组(A组),BrdU标记BMSCs水凝胶复合物;②阳性对照组(B组),BrdU标记BMSCs;③材料组(C组),BrdU标记后水凝胶;④空白组(D组),等量胎牛血清.分别于AMI造模前、AMI造模后1周及心外膜注射处理后4周对4组兔进行超声检查.常规超声心动图测定左室舒张末期内径(LVDd)、左室前壁舒张末期厚度(AW)、左室射血分数(LVEF),定量组织速度成像(QTVI)分析左室前壁、前室间隔基底段、中段收缩期峰值运动速度(Vs)及舒张早期峰值运动速度(VE).结果 与AMI造模前比较,AMI造模后1周4组AMI模型兔LVDd显著增大,AM厚度显著变薄,LVEF显著减低,梗死局部Vs、VE均显著降低,差异有统计学意义(P＜0.05).与AMI造模后1周比较,心外膜注射后4周材料组与空白组LVDd、AM厚度、LVEF及梗死局部Vs、VE无显著变化;处理组与阳性对照组LVDd显著减小,AM厚度显著增厚,LVEF及梗死局部Vs、VE显著增高,差异有统计学意义(P＜0.05),以处理组变化更显著(P＜0.05).结论 骨髓干细胞水凝胶复合物可有效治疗心肌梗死,改善心肌梗死后左室重构及梗死局部收缩、舒张功能.

Study of left ventricular function recovery after bone marrow mesenchymal stem cells with hydrogel composite injection in rabbits with myocardial infarction

Objective To detect cardial function in acute myocardial infarction(AMI)rabbits models before and after bone marrow mesenchymal stem cells(BMSCs)with hydrogel composite injection and assess therapeutic effect of this means.Methods Anterior AMI was produced in fifty-six rabbits and BMSCs with hydrogel composite were prepared.One week after AMI,fifty-two rabbits were divided into 4 groups and accepted epicardial injection:①group of treatment(group A),BMSCs with hydrogel composite marked by BrdU;②group of masculine control(group B),BMSCs marked by BrdU;③group of materials(group C),hydrogel marked by BrdU;④group of blank(group D),fetal bovine serum.Echocardiography were performed before AMI,1 week after AMI and 4 weeks after epicardial injection.The conventional echocardiography indices included left ventricular diameter of end diastole(LVDd),thickness of anterior wall(AW)and left ventricular ejection fraction (LVEF).QTVI indices included the peak velocity in systole(Vs)and peak velocity in early diastole(VE).Results Compared with before AMI,rabbits of all 4 groups had significantly larger LVDd.thiner AW,lower LVEF.lower Vs and VE(P＜0.05)1 week after AMI.Compared with 1 week after AMI,group A and group B had significantly smaller LVDd,thicker AW,larger LVEF,lager Vs and VE(P＜0.05)4 weeks after epicardial injection,which were more significant in group A(P＜0.05);but there was no significant difference in group C and group D.Conclusions BMSCs with hydrogel composite can cure AMI effectively,decrease reconstitution of left ventricle and improve local systolic,diastolic function after AMI.